BLKIM051c Clinical Immunology - practice

Faculty of Medicine
Autumn 2016
Extent and Intensity
0/0/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (lecturer)
Mgr. Petr Bejdák (seminar tutor)
RNDr. Jana Nechvátalová, Ph.D. (seminar tutor)
Mgr. Marcela Vlková, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Miroslava Bučková
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Prerequisites (in Czech)
BLKFY0222p Physiology II-lec.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The practical course is focussed to methods used in laboratory diagnostics of immunopathological states, to indication and interpretation of laboratory immunological test and diagnostic algorithms of immunopathological diseases. After finishing the course the student will be able to apply and interpret basic immunological laboratory tests. The sudents must manage laboraty tests protocol preparation of the tests used.
Syllabus
  • Principles of reaction of antigen plus antiboidy.
  • Agglutination.
  • Precipitation in gel and solution. Immunoelectrophoresis, immunofixation.
  • Immunoassays, imunofluorescence, ELISA, RIA, LIA.
  • Isolation of the cells of the immune system from blod and other biological material.
  • Flow cytometry.
  • Lymphocyte cultivation in vivo, proliferation tests, cytokine production. Tests for in-vitro production of antibodies, ELISPOT.
  • Phagocytic tests.
  • Complement system evaluation.
Literature
  • Vyšetřovací metody v imunologii. Edited by Jiřina Bartůňková - Milan Paulík. 2., přeprac. a dopl. vyd. Praha: Grada, 2011, 164 s. ISBN 9788024735337. info
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
Teaching methods
Laboratory practice. Home preparation for the practice is required.
Assessment methods
To obtain the credit presence in 80% of the lectures is required. The quality of laboratory protocols is assessed.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 10.
The course is also listed under the following terms Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2017, autumn 2018, autumn 2019.
  • Enrolment Statistics (Autumn 2016, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2016/BLKIM051c