aZLHE0221c Histology and Embryology I - practice

Faculty of Medicine
Spring 2017
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Ivana Baltasová (seminar tutor)
prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
MUDr. Jana Dumková, Ph.D. (seminar tutor)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
Mgr. Hana Kotasová, Ph.D. (seminar tutor)
doc. RNDr. Irena Koutná, Ph.D. (seminar tutor)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
MUDr. Veronika Sedláková, Ph.D. (seminar tutor)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Timetable
Thu 9:30–11:20 F01B1/223
Prerequisites (in Czech)
( aZLAN0131s Anatomy I - seminar || ZLAN0131s Anatomy I - seminar ) && ( aZC011 Handling chemical substances || ZC011 Handling chemical substances )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy. 2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue. 3. Identify tissue types and organs of circulatory, lymphatic and respiratory systems in histologic slides. 4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs. 5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis. 6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord. 7. Characterize main phases of prenatal development.
Syllabus
  • 1. Introduction, organization of practicals, teaching aids. Introduction into histological technique. Overview of tissue processing for light and electron microscopy - explanation and film. Study of hard tissues (bones and teeth). (Explanation and film)
  • 2. Cytology: Ultrastructure of the cell nucleus (during interphase and mitosis). Ultrastructure of cell organelles (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes,peroxisomes, and the centriol). Cell inclusions. Cell surfaces and intercellular junctions. Aids: Atlas of electron micrographs.
  • 3. Blood: Blood corpuscles: Cytomorphology of erythrocytes and leukocytes. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Thrombocytes. Slide: A smear of peripheral blood stained by Pappenheim's method. Repetition I: Tissue processing for light and electron microscopy. Cytology (a test). Development of blood cells (hematopoiesis): Erythropoiesis, granulopoiesis and development of thrombocytes. Microscopic structure of the bone marrow. Slide: A bone marrow smear stained by Pappenheim's method.
  • 4. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum).
  • 5. Epithelial tissue - epithelial membranes (covering epithelia). Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip. Glandular epithelium. Absorptive, respiratory and sensory epithelia. Slides: Intestinum tenue, Intestinum crassum, Glandula parotis, Glandula submandibularis, Papilla vallata (taste buds)
  • 6. Connective tissue proper ans special connetive tissues - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification. Repetition II:Blood and development of blood cells, connective and supporting tissues (a test).
  • 7. Muscle tissue: the smooth, akeletal and crdiac. Slides: Intestinum tenue, Intestinum crassum, Glandula parotis, Glandula submandibularis, Apex linguae, Cardiac muscle tissue (H.E. + staining according to Heidenhain)
  • 8. Nerve tissue: neuron, dendrites, neurit and synapses; neuroglia. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
  • 9. Microscopic anatomy and embryology
  • Microscopic structure of the heart and blood vessels. Capillaries. Slides: Artery of the muscular type with a vein, Aorta, Vena cava, Myocardium. Atlas of electron micrographs: Types of capillaries. Repetition III: Epithelial tissue, muscle tissue and nerve tissue (a test).
  • 10. Microscopic structure of the lymphatic system. Slides: Lymphonodus, Lien (spleen), Thymus of a young individual, Thymus of the adult (with involution changes), Palatine tonsil, Lingual tonsil.
  • 11. Microscopic structure of the respiratory system. Slides: Nasal conchae, Epiglottis, Larynx, Trachea, Pulmo.
Literature
    recommended literature
  • MESCHER, Anthony L. Junqueira's basic histology : text and atlas. 13th ed. New York: McGraw-Hill Medical, 2013, xi, 544. ISBN 9781259072321. info
    not specified
  • EROSCHENKO, Victor P. Di Fiore's atlas of histology with functional correlations. Edited by Mariano S. H. di. Fiore. 11th ed. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2008, xvii, 532. ISBN 9781608314928. info
Teaching methods
In the course students observe histological slides and electron micrographs in microscopic hall.
Assessment methods
Testing of knowledge: Student must prove sufficient level of knowledge by written test examination. Each student completes 4 partial tests during semester. Tests are evaluated by point for correct answer. More than half number of correct answers (points) is evaluated as "YES". All of these tests must be successful. In case of failure, only 1 resit is possible. There is condition 4 from 4 (ie. 4 YES / 4 regular tests) or 4 from 5 (ie. 3 YES / 4 regular tests + 1 YES / 1 resit). If student does not fulfill this condition, credit test follows in the relevant exam period. This test covers all topics studied during semester. In case of failure in credit test, credit will not be given and student must enroll the course again.
Conditions for obtaining credit: 1. Attendance at all practical exercises (100% participation, all absences must be regularly excused (in IS) and substituted).
2. Successful completion of all tests.
3. Submission of all protocols (correctly completed forms of protocols signed by teacher).
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.med.muni.cz/histol/vyukac.htm
The course is also listed under the following terms Spring 2016, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2017, recent)
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