BLBC0211c Biochemistry - practice

Faculty of Medicine
spring 2023
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jindra Smutná, Ph.D. (assistant)
Mgr. David Galuška (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Thu 16. 2. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 23. 2. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 2. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 9. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 16. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 23. 3. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 30. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 6. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 13. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 20. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 27. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 4. 5. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 18. 5. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 25. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212
Prerequisites (in Czech)
BLLC0111s Medical Chemistry - seminar && BLLC0111c Medical Chemistry - practice && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the work with biological material and with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and with the basic methods of interpretation of laboratory results.
Learning outcomes
After completing the course, the student will be:
- acquainted with the basic methodological principles and procedures used in the clinical laboratory in determining the concentration of analytes and enzyme activity in body fluids.
- able to follow the principles of working with biological material.
- able to orientate in basic laboratory tasks.
- able to interpret the result of laboratory examination.
Syllabus
  • Introduction to practical exercises - organization, principles of work and safety, principles of pipetting. Blood, laboratory examination.
  • Selected examinations of glucose metabolism - P/U-glucose, OGTT, U-ketone bodies, S-HbA1
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol.
  • Selected liver tests - S-ALT/AST, S/U-bilirubin, U-UBG, S-albumin
  • Selected examinations of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S/B-CRP, S-ferritin
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, U-blood, S/U-urea, nitrogen balance
  • Laboratory diagnosis of myocardial infarction - B-troponin T, diagnostic accuracy
  • Chemical qualitative analysis of urine - diagnostic strips
  • Selected examinations of urolithiasis - S/U-uric acid, indicative chemical analysis of stones
  • Selected examinations of the digestive tract - examination of the stomach, pancreatic disease, FOB
Literature
    required literature
  • Studijní podklady a materiály k předmětu dostupné v IS MUNI.
Teaching methods
Lab exercises
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes, missed exercises must be made up,
  • elaboration and approval of all protocols,
  • completion of the final test (at least 70% success rate) or the credit test (at least 60% success rate).
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu BLBC0211p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2024, spring 2025.
    • Enrolment Statistics (spring 2023, recent)
    • Permalink: https://is.muni.cz/course/med/spring2023/BLBC0211c