CH2BP_6P5L Biochemistry Laboratory Training

Faculty of Education
Spring 2015
Extent and Intensity
0/0/2. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Petr Ptáček, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Sládek, CSc.
Department of Physics, Chemistry and Vocational Education – Faculty of Education
Contact Person: Mgr. Bc. Lucie Viková
Supplier department: Department of Physics, Chemistry and Vocational Education – Faculty of Education
Timetable of Seminar Groups
CH2BP_6P5L/01: Tue 8:25–10:05 laboratoř 89, P. Ptáček
CH2BP_6P5L/02: Thu 16:40–18:20 laboratoř 89, P. Ptáček
CH2BP_6P5L/03: Thu 8:25–10:05 laboratoř 89, P. Ptáček
Prerequisites
CH2BP_2P2L Laboratory Technics || CH2BP_3P3L Laboratory Technics
Theoretical knowledge of the issue.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives can be summarized as follows:
to practically prove the basic characteristics of the biochemical compounds and methodical advancements for its study and enzyme analytical improvement
practical performance and prove of qualitative and quantitative reactions of carbohydrate, lipids, amino-acids, proteins, nucleic acids and enzymes
to practically and expertly present own laboratory work and results
Syllabus
  • Qualitative reactions of carbohydrates
  • Carbohydrate thin-layer chromatography
  • Preparation and identification of lipids in egg yolk
  • Verification and chromatography separation of leaf pigments
  • Characteristic reactions of amino acids and proteins
  • Verification of amino acids and peptide
  • Amino acids paper chromatography
  • Separation of proteins
  • Separation and properties of nucleic acids
  • Enzyme reaction temperature dependency
  • Verification of proteins
  • Ezyme substrate specification
  • Enzyme activity determination
  • Kinetics of enzyme reaction
Literature
  • ŠIBOR, Jiří and Hana CÍDLOVÁ. Praktická cvičení z biochemie a bioorganické chemie (Hands-on Training of Biochemistry and Bioorganic Chemistry). 1. vydání. Brno: Masarykova univerzita v Brně, 2005, 49 pp. ISBN 80-210-3883-7. info
Teaching methods
Teaching type: laboratory practice
Assessment methods
Requirements for completion: laboratory practice acquittance
elaboration of laboratory protocols
credit
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2016, Spring 2017, Spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2015, recent)
  • Permalink: https://is.muni.cz/course/ped/spring2015/CH2BP_6P5L