C7021 Separation Methods

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Milan Dressler, CSc. (lecturer)
doc. Mgr. Jan Havliš, Dr. (lecturer)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Milan Dressler, CSc.
Chemistry Section – Faculty of Science
Prerequisites
C3100 + C4050 Analytical chemistry (at least C1660)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Basics of analytical separation methods, transit from liquid-liquid extraction to chromatography. Theory and praxis of Gas chromatography (GC). Efficiency of separation methods. Ion exchange chromatography. Sample preparation for analytical separation.
Syllabus
  • Principles of gas chromatography, diffusion in gas phase, description of compound distribution between phases and character of compounds interaction in stationary and mobile phases. The influence of temperature and pressure of gas characteristics in gas chromatography: van Deemter theory. Basic components of gas chromatograph, sample injection. Thermo conductivity detection in gas chromatography (katharometer). Flame ionization detection (FID). Electron capture detector (ECD). Photoionization detector, conductivity detector. Types of columns in gas chromatography, stationary phase support. Capillary columns in gas chromatograhy, influence of inner diameter of capillary and the film thickness on separation. Adsorption gas chromatography - common adsorbents. Adsorbed and covalently bonded stacionary phases in gas chromatography - their properties, preparation and selection. Requirements for gas chromatography columns. Gas chromatography with programmed temperature - purpose, temperature influence on separation and characterization of retention. Hyphenation of gas chromatography with mass spectroscopy - advantages and applications. Areas of applications and examples of applications of gas chromatography. Head space analysis. Ionic compounds separation - principles. Ion exchange chromatography (IEC). Ionexes. Ion compounds separation - applications. Dionex system. Head space analysis. Supercritical fluid chromatography (SFC). Sample preparation. Thin Layer chromatography (TLC). Efficiency of separation methods. Liquid-liquid extraction. Accelerated solvent extraction (ASE). Microwave extraction.. Solid phase extraction (SPE). Solid phase microextraction (SPME). Supercritical fluid extraction (SFE).
Literature
  • CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
  • POPL, Milan and Jaroslav KUBÁT. Separace látek. 3. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1986, 171 s. info
  • SOMMER, Lumír. Základy teorie analytické chemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1985, 250 s. info
  • SOMMER, Lumír. Základy teorie analytické chemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1990, 138 s. ISBN 8021001070. info
  • SOMMER, Lumír. Základy teorie analytické chemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1991, 185 s. ISBN 8021002158. info
  • Separační metody - Plynová chromatografie
Assessment methods (in Czech)
přednáška Zk
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.chemi.muni.cz/~pazourek/student/sepmetody.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2002, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2002/C7021