C3181 Biochemistry I

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites
C2021 Organic Chemistry I || C2700 Basic Organic Chemistry
Basic knowledge of organic and general chemistry, biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Basic properties of natural substances. Aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site. Thermodynamics. Enzyme kinetics, inhibition, allostery. Regulation of enzyme acitivity.
Syllabus
  • 1. Aminoacids, properties, determination, chromatography 2. Peptides, peptide bond, primary, secondary, terciary, quaternary structure 3. Non-repetitive structures, colagene, hydropathy, properties of proteins 4. Glycides, properties, reactions, anomers, semiacetals, anomers. conformation 5. Glycosides, oligosaccharides, polysaccharides (starch, glycoge, chitin), heteropolysaccharides. 6. Lipids, acylglycerols, phospholipids, plasmalogens, sphingolipids, steroids 7. Nucleic acids, nucleosides, nucleotides, primary structure 8. DNA, RNA, double-helix, superhelix, denaturation, hybride structure, methods for determination of primary structure 9. Thermodynamics in biochemistry, macroergic bond, reaction kinetics, enzymes, their active site, prosthetic groups, coenzymes, mechanism of enzyme catalysis 10. Coenzymes and vitamins, nicotinamide and NAD, flavins, ATP. AMO, cAMO, biotin, thiamin, coenzyme A, lipoic acid, folic acid 11. Pyridoxalphosphate, B12, metaloporphyrins, iron-sulfur proteins, vitamin C, lipophilic vitamins 12. Michaelis-Menten equation, Km, VL, turnover number, enzyme activity 13. Anabolims, catabolism, their regulation, anaerobic glycolysis 14. Gluconeogenesis, synthesis of PEP, glycogenolysis, synthesis of glycogen, Cori cycle
Literature
  • Vodážka - Biochemie
  • Voet, Voetová - Biochemie; Zubay, Parson, Vance - Principles of Biochemistry
  • Mikeš - Základní pojmy z biochemie II
Assessment methods (in Czech)
Základní přednáška 2h, doplněná seminářem 1h, navazuje základní biochemické praktikum
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.