C7880 Separation Methods II

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
doc. RNDr. Oldřich Janiczek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
( C4182 Biochemistry II || C3580 Biochemistry ) && ( C6200 Biochemical Methods || C6260 Protein Separation || C4830 Inst. Methods in Biochemistry )
Basic knowledges from general biochemistry and methods of biochemical research.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goals of the lecture are to get to students the knowledge from separation methods in biochemistry and molecular biology, which are used for macromolecules purification (proteins, nucleic acids). A content of the first part includes electromigration methods. Second part is oriented onto chromatography methods and third part is dedicated in Field Flow Fractionation, Supercritical Fluid Chromatography and Mass Spectrometry.
Syllabus
  • A.Elektromigration Methods
  • 1.Capillary Zone Electrophoresis
  • 2.Izotachophoresis
  • 3.Detection of Protein after Electrophoresis or Isoelectric Focusing
  • 4.Blotting
  • 5.Electrohpresis od Nucleic Acids
  • B.Field Flow Fractionation
  • C.Chromatography Methods
  • 1.Microcolumn Chromatography
  • 2.Gas Chromatography
  • 3.Supercritical Fluid Chromatography
  • D.Mass Spectrometry
  • E.Application of Affinity Interaction in Protein Purification
Literature
  • SNYDER, Lloyd R., Joseph Jack KIRKLAND and Joseph L. GLAJCH. Practical HPLC method development [Snyder, 1997]. 2nd ed. New York: John Wiley & Sons, 1997, xxvi, 765. ISBN 0-471-00703-X. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
  • WEISS, Joachim. Ion chromatography. 2nd ed. Weinheim: VCH Verlagsgesellschaft, 1995, 465 s. ISBN 3-527-28698-5. info
  • ROTHE, Gunter M. Electrophoresis of enzymes : laboratory methods. Berlin: Springer-Verlag, 1994, 307 s. ISBN 3540581146. info
  • Handbook of capillary electrophoresis. Boca Raton: CRC Press, 1994, 649 s. ISBN 0-8493-8690-X. info
  • Protein blotting : a practical approach. Edited by Bonnie S. Dunbar. Oxford: Oxford University Press, 1994, 242 s. ISBN 0199634378. info
  • KUHN, Reinhard and Sabrina HOFFSTETTER-KUHN. Capillary electrophoresis : principles and practice. Berlin: Springer-Verlag, 1993, x, 375 s. ISBN 3-540-56434-9-. info
  • LI, Sam F. Y. Capillary electrophoresis : principles, practice and applications. Amsterdam: Elsevier, 1993, 582 s. ISBN 0444815902. info
  • FORET, František, Ludmila KŘIVÁNKOVÁ and Petr BOČEK. Capillary zone electrophoresis. Weinheim: VCH Publishers, 1993, 346 s. ISBN 3-527-30019-8. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • ANDREWS, Anthony T. Electrophoresis : theory, techniques and biochemical and clinical applications. 1st pub. Oxford: Clarendon Press, 1990, xv, 452 s. ISBN 0-19-854632-7. info
  • Gel electrophoresis of proteins : a practical approach. Edited by B. D. Hames - D. Rickwood. 2nd ed. Oxford: Oxford University Press, 1990, xviii, 383. ISBN 0199630755. info
  • High performance liquid chromatography in biotechnology. Edited by William S. Hancock. New York: John Wiley & Sons, 1990, 564 s. ISBN 0471825840. info
  • MIKEŠ, O. High-performance liquid chromatography of biopolymers and biooligomers. P. B, separation of individual compound classes. Amsterdam: Elsevier, 1988, 721 s. ISBN 0444430342. info
Assessment methods (in Czech)
Zkouška probíhá písemnou formou. Studenti dostávají test zahrnující otázky z přednášených technik separace makromolekul. Doba testu je 1 hodina. Klasifikace je A-F respektive "prospěl" nebo "neprospěl".
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught once in two years.
Information on the per-term frequency of the course: (liché roky).
The course is taught: every week.
Teacher's information
http://orion.chemi.muni.cz/biochem/student.htm
The course is also listed under the following terms Autumn 1999, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2011, Autumn 2011 - acreditation, Autumn 2014, Autumn 2016, Autumn 2018, Autumn 2020, Autumn 2022, Autumn 2024.