Bi9250 Special Immunological Methods

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Alena Žákovská, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Alena Žákovská, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Žákovská, Ph.D.
Timetable
Tue 10:00–11:50 BR2
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
  • General Biology (programme PřF, N-BI, specialization Physiology of Animals)
Course objectives
Survey of most significantly contemporary immunological methods using in immunological laboratories. Excursions in chosen workplaces. Main objectives can be summarized as follows: to understand the basic immunological technics; to learn the special immunological methods used in research; At the end of this course, students should be able to: understand, be able to explain and formulate the most important current immunological methods used in immunological labs and in research; make reasoned decisions about immunological pro blems in diagnosis; His obtained data can be able to use in practical labs and praxis.
Syllabus
  • 1. lecture: organizaction of lectures and practice course; Methods used in clinical praxis; Sérological method (precipitation, immunodiffusion, immunoelectrophoretic, agglutination hemagglutination, complement methods, immunoblotting)
  • 2. lecture: elongation of serological methods, nephelometry, turbidimetry
  • 3. lecture: immuno-chemical methods, RIA, FIA, EIA
  • 4. lecture: EIA method in detail, ELISA
  • 5. lecture: ELISA method in detail, different kinds of ELISA, workshop of home-made ELISY
  • 6. lecture: a) work with the immune system cell, the determination of leukocyte populations and their function b) special in vitro tests- tests for examination of leukocyte populations and their function, tests of lymphocyte proliferationci, test of lymphocyte transphormation test, investigation of HLA antigens
  • 7. lecture: Alergological investigation
  • 8.lecture: Základy interpretation of the results of immunological lab tests, metods used in clinical labs,
  • 9. lecture: 9.-12. lectures: Excursion in labs:
Literature
  • Doporučená literatura k předmětu Speciální imunologické metody – přednáška a cvičení
  • Doporučená literatura k předmětu Speciální imunologické metody – přednáška a cvičení
Assessment methods
The students participation at lectures is demanded. The final clafficiation will be in the form of written test at the end of term .
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi9250