Bi6888 Protein Design and Engineering

Faculty of Science
Autumn 2009
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. Mgr. Radka Chaloupková, Ph.D. (lecturer)
prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Jiří Damborský, Dr.
Timetable
Mon 8:00–9:50 C04/211
Prerequisites
ability to communicate in English
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 25 student(s).
Current registration and enrolment status: enrolled: 0/25, only registered: 0/25, only registered with preference (fields directly associated with the programme): 0/25
fields of study / plans the course is directly associated with
Course objectives
The students will learn various aspects of research and development focused on construction of proteins by rational design and directed evolution in this seminar. Theoretical and experimental problems of protein engineering are being discussed, including transfer of the results from research to practice. Microbial enzymes haloalkane dehalogenases serve as the model system for practising of various protein engineering strategies. The students refer once a week about the results of their own research projects as well as the results published in scientific literature within so called "Journal Clubs". They are supervised to critically access content and formal aspects of presentations. The students are being informed about hot news in the field presented at scientific conferences and refer about the meetings they have attended during the year. The students get first experience with chairing scientific discussions and learn how to prepare minutes from the meeting.
Syllabus
  • Screening of New Proteins by Bioinformatic Analysis
  • Rational Design of Proteins by Computer Modelling
  • Construction of Modified Proteins by Site-Directed Mutagenesis and Directed Evolution
  • Characterization of Modified Proteins by Biochemical and Biophysical Techniques
Literature
  • Protein Structure and Function, Gregory A. Petsko, Dagmar Ringe, New Science Press, 2004 ISBN 1405119225, 9781405119221, str. 195
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: registration to this course must be approved by teachers
The course is also listed under the following terms Spring 2011 - only for the accreditation, Autumn 2010 - only for the accreditation, Autumn 2008, Spring 2009, Spring 2010, Autumn 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014.
  • Enrolment Statistics (Autumn 2009, recent)
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