PřF:C7999 Advanced Methods of NMR - Course Information

C7999 Advanced Methods of NMR Spectroscopy

Extent and Intensity

0/0/2. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Radek Marek, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Radek Marek, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. RNDr. Radek Marek, Ph.D.

Prerequisites

Students should have knowledge of NMR at the level of the courses: NMR Structural Analysis C8950 or Theoretical Concepts of NMR C5320

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 3 student(s).
Current registration and enrolment status: enrolled: 0/3, only registered: 0/3, only registered with preference (fields directly associated with the programme): 0/3

fields of study / plans the course is directly associated with

• Analytical Chemistry (programme PřF, M-CH)
• Analytical Chemistry (programme PřF, N-CH)
• Inorganic Chemistry (programme PřF, M-CH)
• Inorganic Chemistry (programme PřF, N-CH)
• Biomolecular Chemistry (programme PřF, N-BCH)
• Physical Chemistry (programme PřF, M-CH)
• Physical Chemistry (programme PřF, N-CH)
• Macromolecular Chemistry (programme PřF, N-CH)
• Material Chemistry (programme PřF, N-CH)
• Organic Chemistry (programme PřF, M-CH)
• Organic Chemistry (programme PřF, N-CH)
• Structural Chemistry (programme PřF, N-CH)

Course objectives

At the end of the course students should be able to develope advanced methods of 1D and 2D NMR spectroscopy of small molecules. The course is focused on hands-on experience of modern techniques in small molecule NMR spectroscopy.

Syllabus

• 1. Basic 1D NMR spectra: pulse sequence, acquisition parameters, pulse calibration (direct and indirect), probehead exchange 2. Processing and parameters, temperature measurement 3. Homonuclear 2D chemical shift correlation experiments: ¹H-¹H COSY, ³¹P-³¹P COSY, MQF-COSY, NOESY 4. Heteronuclear 2D chemical shift correlation experiments: ³¹P-¹⁹F HETCOR, COLOC 5. Inverse (proton) detection: HSQC, HMQC, HMBC, pulsed-field gradients 6. Practical aspects for measuring various nuclei: 15N nucleus (direct and indirect detection), nucleus with spin > ¹/₂ (¹⁴N, ¹⁷O), ²⁹Si, ⁷⁷Se, ¹⁹⁵Pt 7. Selective and shaped pulses, combined (hybrid) experiments: selective NOE, HSQC-TOCSY

Literature

• Claridge, Timothy D.W. High-Resolution NMR Techniques in Organic Chemistry, Amsterdam, Pergamon, 1999, ISBN 0-08-042798-7
• Braun, S. - Kalinowski, H.O. - Berger, S. 100 and More Basic NMR Experiments, Weinheim, VCH, 1996, ISBN 3-527-29091-5

Teaching methods

Laboratory training and class discussion.

Assessment methods

Presence in practical sessions in NMR laboratory is required. Final examination will be based on the structural analysis of unknown sample by using 2D NMR techniques.

Language of instruction

English

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (Autumn 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2009/C7999