C7195 Advance laboratory course of biochemistry

Faculty of Science
Autumn 2010
Extent and Intensity
0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.
Timetable
Wed 8:00–11:50 C05/108
Prerequisites
C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.
Syllabus
  • Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein
Literature
  • Návody ke cvičením - viz Studijní materiály
Teaching methods
Laboratory course
Assessment methods
Credit course
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2010/C7195