C7910 C7910 Chemical Research Methods

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Fri 9:00–10:50 C05/114
Prerequisites
C6200 Biochemical Methods
Biochemistry I, II. Physical Chemistry I, II or Backgrounds of Physical Chemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand and explain these topics - Principles of chemical and physical methods used in biochemical research. Their instrumentation and use in routine analysis and study of biochemical processes. Metodological approaches in study of biomolecules behaviour and their interconversions.
Syllabus
  • 1. Introduction. Principles of the work with biological material. Strategy and planning. Desintegration of tissues and cells.
  • 2. Preparative centrifugation. Sedimentation analysis.
  • 3. Phase separations. Precipitation and extraction.
  • 4. Membrane separations. Concentration and drying. Water purification.
  • 5. Chromatographic methods. General principles and characte- ristics. Adsorption and distribution chromatographies.
  • 6. Ion exchange chromatography, chromatofocusation. Gel and affinity chromatographies. Gas chromatography.
  • 7. Mass spectrometry.
  • 8. Electromigration methods. General characteristics and side effects. Electrophoresis, its modifications. Isotachophoresis.
  • 9. Immunochemical methods.
  • 10. Spectral methods. General characteristics, classification. Electronic spectra of atoms. AES and AAS. Applications in biochemistry.
  • 11. Electronic spectra of molecules.
  • 12. UV-VIS spectroscopy, principles, use.
  • 13. Types of instruments, difference spectra, perturbation. Turbidimetry and nefelometry.
  • 14. Luminiscence methods, principles. Spectrofluorometry, principles, use.
  • 15. Biochemical applications, fluorescence polarisation, probes.
  • 16. IR and Raman spectroscopy.
  • 17. Moessbauer spectra.
  • 18. NMR, biochemical applications. EPR, biochemical applications.
  • 19. Chiroptic methods. ORD and CD. Principle, meaning and applications in biochemistry.
  • 20. Calorimetry, types of instruments, biochemical applications.
  • 21. Methods of determination of the size and shape of macromole- cules. Isotope exchange.
  • 22. Coligative methods, osmometry. Viscosimetry.
  • 23. Translational and rotational diffusion.
  • 24. Light and X-rays scattering, rentgenostructure analysis.
  • 25. Electrochemical methods in biochemistry. Principles, classification. Potentiometry, ISE. Bioselective electrodes.
  • 26. Amperometric methods. Polarography and voltammetry, bio- sensors.
  • 27. Fast reactions, metods, biochemical applications.
  • 28. Ligand binding on macromolecules, types, methods of determination, calculations.
Literature
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986, 199 s. info
  • KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987, 430 s. URL info
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2., přeprac. a rozš. vyd. Praha: Academia, 2000, 337 s. ISBN 8020007911. info
Teaching methods
Theoretical preparation.
Assessment methods
Lecture. Written exmination event. concuring oral exam.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught once in two years.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, autumn 2017, Autumn 2020.
  • Enrolment Statistics (Autumn 2010, recent)
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