Bi8980c Protein Preparation and Characterization I - practice

Faculty of Science
Autumn 2011
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.
Prerequisites
NOW( Bi8980 Protein preparation )
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The practical course intended for students in the early stages of their research career, will deal overexpression of proteins in prokaryotic systems with different expression vectors, strategy of protein purification and their characterisation. At the end of this practical course the student will be able to: use the bioinformatics in analysis and planning of a protein expression project; design primers and optimize gene expression; compare in vectors containing different tags and fusion proteins and different strains; optimize the quality of your favorite protein during different purification strategies.
Syllabus
  • 1.Strategy of cloning into expression plasmids. 2. Expression and purification of the human scFv produced in cytoplasm or periplasmic space. 3. Expression and purification of the plant protein (A. pET32 – thioredoxin - chaperon/B. pRSET – His.tag/ C. pET161DEST – Gate way technology – Lumio.tag/D. pET28 – without any tag.)
Literature
    recommended literature
  • PRICE, Nicholas C. and Jacqueline NAIRN. Exploring proteins : a student's guide to experimental skills and methods. Oxford: Oxford University Press, 2009, xv, 512. ISBN 9780199205707. info
Teaching methods
Practical course
Assessment methods
protocol, written test
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: blok 5 dnů.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/Bi8980c