C8980c Protein Preparation and Characterization I - practice

Faculty of Science
Autumn 2014
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
RNDr. Lubomír Janda, Ph.D. (lecturer)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
C8980 Protein preparation
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for registration for the course. Students must pass this course (C8980 Expression and purification of proteins) before registration for course C8980c.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After completion of the course, the student will posess basic practical knowledge of how to express and purify recombinant proteins, will have experience with various expression systems and will be able to use different purification methods. During the course the student will be acquainted with the various methods of detection and is able to mark proteins for NMR analysis or crystallography.
Syllabus
  • 1. Strategy of cloning into expression plasmids. 2. Expression and purification of the human scFv produced in cytoplasm or periplasmic space. 3. Expression and purification of the plant protein (A. pET32 – thioredoxin - chaperon/B. pRSET – His.tag/ C. pET161DEST – Gate way technology – Lumio.tag/D. pET28 – without any tag.)
Literature
    recommended literature
  • PRICE, Nicholas C. and Jacqueline NAIRN. Exploring proteins : a student's guide to experimental skills and methods. Oxford: Oxford University Press, 2009, xv, 512. ISBN 9780199205707. info
    not specified
  • Protein purification : principles, high resolution methods, and applications. Edited by Jan-Christer Janson. 3rd ed. Hoboken, N.J.: John Wiley & Sons, 2011, xiv, 517. ISBN 9780471746614. info
  • HARDIN, Charles. Cloning, gene expression and protein purification : experimental procedures and process rationale. New York: Oxford University Press, 2001, viii, 435. ISBN 0195132947. info
  • Guide to protein purification. Edited by Richard R. Burgess - Murray P. Deutscher. 2nd ed. Boston: Elsevier/Academic Press, 2009, liii, 851. ISBN 9780123749789. info
Teaching methods
The main teaching method are laboratory exercises which follows the lecture series of the subject called Protein expression and purification (C8980).
Assessment methods
The course is successfully concluded after completion of the block exercises and submitting laboratory protocols that were approved by the teacher.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: blok 5 dnů.
The course is also listed under the following terms Autumn 2012, Autumn 2013, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2014, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2014/C8980c