Bi7340 Cytology and morphology of bacteria

Faculty of Science
Autumn 2018
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Iva Buriánková, Ph.D. (seminar tutor)
Mgr. Lukáš Fidrich (assistant)
Ing. Jiřina Kučerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Iva Buriánková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi7340/01: Mon 17. 9. to Fri 14. 12. Wed 13:00–14:50 E25/111, I. Buriánková
Bi7340/02: Mon 17. 9. to Fri 14. 12. Wed 15:00–16:50 E25/111, I. Buriánková
Prerequisites
NOW( Bi7330 Cytology and morphology of bacteria )
general microbiology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16, only registered with preference (fields directly associated with the programme): 0/16
fields of study / plans the course is directly associated with
Course objectives
Students will be able consider the sutiable technique for observing microscopic and macroscopic characteristics of bacteria and their morphological diversity (including the cell morphology of particular bacterial species depending on cultivating media) and observing structures of bacterial cells (endospores, inclusions, flagellum). Student will be able consider the applicability of particular methods for clinical, environmental and biotechnological practice. Student will be able of preparing of variable stained and native microscopic slides, will be able operate variable microscopical techniques (phase and Nomarsky contrast, bright field microscopy) and handle with images acquired by CCD camera, photographic camera and analyse them (image acquisition - LUCIA G).
Syllabus
  • Morphology of bacterial and archaeal cells and colonies, basic staining methods.
  • Techniques of work with light microscope, care, removing of simple problems.
  • Microphotography, digital photography, the use of video-cameras in image acquisition in microbiology, computer image analysis.
  • Determination of the size of bacterial cells and their morphological structures (the use of ocular micrometer and software for image analysis).
  • Gram staining. Acid-fast bacteria, Ziehl-Neelsen staining, mycobacteria. Giemsa staining.
  • Endospores and sporulation of bacteria.
  • Phase contrast and Nomarski differential interference contrast.
  • Capsules, sheaths and slimes of bacterial cells.
  • Movement of bacterial cells.
  • Technique of slide cultures.
  • Actinomycetes and methods of their morphology observation.
  • Measurement and comparison of proportions of selected groups of microorganisms.
  • Staining of cell wall, nuclear material and reserved compounds in cells of bacteria and yeasts.
Literature
  • PERRY, Jerome J. and James T. STALEY. Microbiology :dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0-03-053893-9. info
  • Laboratory experiments in microbiology. Edited by Ted R. Johnson - Christine L. Case. 4th ed. Redwood City: Benjamin Cummings, 1995, xi, 418 s. ISBN 0-8053-8509-6. info
Teaching methods
laboratory course, outcomes discussion, electronical protocol
Assessment methods
practical documentation of laboratory courses, individual preparing of electronical protocols, discussion about individual results, written exam, examination preparations
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/Bi7340