C8170 Enzymology - seminar

Faculty of Science
Autumn 2018
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 1. 10. to Fri 14. 12. Tue 10:00–11:50 B11/335
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/C8170