PřF:C7920 Struct. and func. of proteins

C7920 Structure and functions of proteins

Faculty of Science
Autumn 2020

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature