PřF:Bi7420 Genome analysis methods - Course Information

Bi7420 Modern methods for genome analysis

Extent and Intensity

1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).

Teacher(s)

MVDr. Boris Tichý, Ph.D. (lecturer)
Mgr. Vojtěch Bystrý, Ph.D. (lecturer)
prof. MUDr. Mgr. Marek Mráz, Ph.D. (lecturer)
Mgr. Lenka Humpolíková Adámková, Ph.D. (lecturer)
Mgr. Terézia Kurucová (lecturer)
Mgr. Filip Pardy (lecturer)
Bc. Jana Jedličková (assistant)

Guaranteed by

MVDr. Boris Tichý, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Vojtěch Bystrý, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Mon 15:00–16:50 B11/305

Prerequisites (in Czech)

Bi1700 Cell Biology && ( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi4020c Molecular biology - practice || Bi6400 Methods of molecular biology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 40 student(s).
Current registration and enrolment status: enrolled: 2/40, only registered: 0/40, only registered with preference (fields directly associated with the programme): 0/40

fields of study / plans the course is directly associated with

• Bioanalytical Laboratory Diagnostics in Medicine - Medical Genetics and Molecular Diagnostics (programme PřF, N-LGM)
• Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Molecular Biology and Genetics (programme PřF, N-MBG)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the course students should get familiar with the modern molecular-biology techniques and explain the principles of microarray and next generation sequencing technologies

Learning outcomes

After participating on the course student will be able to - apply CRISPR technology in his research - describe principle of next generation sequencing (NGS) - design NGS experiment - analyze NGS data

Syllabus

• 1. Microarrays - technological aspects: expression, CGH, SNP arrays, resequencing and protein arrays; 2. Next generation sequencing (NGS) – technological aspects of different NGS platforms 3. Library preparation for NGS 4. Methods for non-coding RNA analysis – miRNA, lncRNA, examples of applications in oncology 5. Data analysis – bioinformatics and statistics 6. Aplications and experiment design

Literature

• Koubková L, Vojtěšek B, Vyzula R. Next generation sequencing -  application in clinical practice. Klin Onkol. 2014;27 Suppl 1:S61-8.

Teaching methods

Lectures.

Assessment methods

written test

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/Bi7420