C6230 Clinical Biochemistry - laboratory course

Faculty of Science
autumn 2021
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Jitka Kašparovská, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
C6230/01: Tue 9:00–12:50 C15/212
C6230/02: Tue 13:00–16:50 C15/212
Prerequisites (in Czech)
C6220 Clinical Biochemistry ||NOW( C6220 Clinical Biochemistry )
Předpokladem zápisu je současné absolvování předmětu klinická biochemie (C6220). K zápisu je potřeba požádat o výjimku-souhlas učitele. Souhlas bude udělován v pořadí: Aplikovaná biochemie (předmět je povinný), Biochemie (předmět je povinně volitelný), ostatní.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 48 student(s).
Current registration and enrolment status: enrolled: 0/48, only registered: 0/48, only registered with preference (fields directly associated with the programme): 0/48
fields of study / plans the course is directly associated with
Course objectives
The laboratory course is designed to introduce the student to the principles and practices of clinical chemistry.
Learning outcomes
Through laboratory experiences the student will develop the necessary skills to perform analytical procedures and evaluate laboratory data. The exercises familiarize the student with the performance of simple laboratory tests common in health care centers.
At the end of the course students should be able to:
  • understand basic principles of basic analytical parameters determination in blood serum and urine;
  • determine basic biochemical parameters in serum and urine;
  • interpret measured results in context of potential health/disease.
    • Syllabus
    • Principles of laboratory biosafety and biosecurity. Collection and processing of blood/urine. Interpretation of results. Biochemical analyzers.
    • Determination of triglycerides. Determination of cholesterol (total, HDL) in serum and blood. Calculation of LDL-cholesterol and derived parameters.
    • Determination of glucose in blood and serum. Oral glucose tolerance test (OGTT). Determination of glycated hemoglobin. Determination glucosuria and ketonuria.
    • Determination of total protein in serum. Electrophoresis of serum proteins. Determination of serum albumin. Determination of serum proteins by ELISA. Determination of cardiomarkers by POCT. Estimation of C-reactive protein.
    • Determination of catalytic concentration of serum enzymes (ALT / AST, ALP isoenzymes, total CK, CK-MB) by kinetic and constant time methods, influence of temperature. Determination of the mass concentration of CK-MBmass.
    • Determination of nitrogen metabolites in serum/urine (total bilirubin, conjugated, uric acid, urea). Approximate evaluation of nitrogen balance.
    • Analysis of urinary proteins (proteinurias, albuminuria, Bence-Jones protein). Chemical dipstick testing.
    • Objective and physical examination urine. Determination of urine osmolality. Determination of serum/urine creatinine. Creatinine clearance and estimation of glomerular filtration rate. Fractional excretion and reabsorption of water.
    • Indicative chemical analysis of urinary stones.
    • Laboratory tests of saliva (rate of salivation, buffering capacity, lactate) Examination of gastrointestinal tract Laboratory examination of stomach (gastric acid output, confirmation of Helicobacter pylori by breath and urease tests). Laboratory test in pancreatic disease (α-amylase, secretin-CCK and NBT-PABA and MTG breath tests). Test of occult bleeding in GIT.
    • Solution of model cases.
    Literature
      required literature
    • Podklady pro jednotlivá cvičení ve Studijních materiálech předmětu C6230 v IS MU
    • Rajdl. D. a kol. Clinical biochemistry (e-book, formats: .pdf, .epub, .mobi) 1st ed. Prague, Karolinum Press, 2016, 426 s. ISBN: 978-80-246-3497-5. Download: https://karolinum.cz/en/books/rajdl-clinical-biochemistry-17096
      recommended literature
    • RACEK, Jaroslav. Klinická biochemie. 2., přeprac. vyd. Praha: Galén, 2006, 329 s. ISBN 8072623249. info
    Teaching methods
    laboratory courses, elaboration of laboratory protocols, discussion, written verification of partial knowledge
    Assessment methods
    Credit. The condition for granting the credit is the completion of all exercises, incl. processing of protocols and at the same time successful completion of the credit test. The credit test can be repeated only once.
    Language of instruction
    Czech
    Further Comments
    Study Materials
    The course is taught annually.
    Teacher's information
    https://is.muni.cz/auth/el/1431/podzim2021/C6230/
    The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.
    • Enrolment Statistics (autumn 2021, recent)
    • Permalink: https://is.muni.cz/course/sci/autumn2021/C6230