C7230 Fluorescence methods in life sciences - a journey from molecules to cells

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Tue 13:00–14:50 C02/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The lecture course is focused on acquiring theoretical knowledge needed for implementation of modern spectroscopic methods for analysis of protein-protein and protein-DNA interactions. From the methodical approaches, the main emphasis is put on highly sensitive fluorescent methods and their practical use in biological laboratories. The main lecture topics are detection, techniques for fluorescent labeling of macromolecules and their application for analysis of function and interaction of important biomolecules in genome and proteome.
Learning outcomes
Student will be able to:
- describe spectral properties of a fluorophore
- use fluorescence for qualitative and quantitative analysis of biomolecular interactions
- design optimal experimental arrangements for fluorescence spectroscopy and microscopy
Syllabus
  • Definition and characteristics of absorption, fluorescence and phosphorescence
  • Instrumentation - spectrophotometer, spectrofluorometer
  • Steady-state fluorescence
  • Time-resolved fluorescence
  • Fluorescence anisotropy and its change after interaction of macromolecules
  • Absorption spectroscopy in biological analysis of molecules
  • Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules
  • Intrinsic fluorescence of biologically important molecules
  • Extrinsic fluorescence – fluorescent labels, probes, and indicators
  • Fluorescence labeling of DNA and proteins
  • Fluorescence microscopy
  • Analytical applications of fluorescence for concentration determination
  • Examples of fluorescence application in biology
Literature
  • Lakowicz J.R.: Principles of Fluorescence Spectroscopy. Third Edition, Springer + Business Media, New York, 2006.
Teaching methods
Theoretical lectures with practical demonstrations
Assessment methods
written test + oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
https://is.muni.cz/auth/el/1431/podzim2016/C7230/um/web/index.html
The course is also listed under the following terms Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (autumn 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2021/C7230