C9035 Evolutionary and comparative plant cytogenetics - practice

Faculty of Science
Autumn 2022
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
prof. Mgr. Martin Lysák, Ph.D., DSc. (seminar tutor)
RNDr. Terezie Malík Mandáková, Ph.D. (seminar tutor)
Mgr. Michaela Kubová (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. Mgr. Martin Lysák, Ph.D., DSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
Prerequisites: basic knowledge in the field of cytogenetica and cytology (e.g., pipetting, preparation of solutions, microscopy).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
The participant will master advanced techniques of plant cytogenetics (microscopic preps, advanced fluorescence microscopy, chromosome painting, genomic in situ hybridization, image processing).
Learning outcomes
Student will be able to:
- prepare micrscopic preparations;
- label isolated DNA by haptens and fluorochromes;
- prepare DNA probes;
- carry out fluorescence in situ hybridization (FISH) of the labeled DNA probes to chromosomes and cell nuclei;
- analyze preparations using fluorescence microscopy;
- photograph chromosomes and cell nuclei;
- analyze and process the acquired photographs.
Syllabus
  • Introduction to evolutionary and comparative plant cytogenetics. Principles of light and fluorescence microscopy. Preparation of mitotic and meiotic chromosomes from roo tip meristems and anthers, microscopic analysis of chromosome preparations. Direct and indirect labelling of DNA probes by nick translation. Fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), comparative chromosome painting (CCP). Immunodetection indirectly labelled DNA probes. Fluorescence microscopy of chromosome preps after FISH, GISH a CCP. Processing of microscopy photographs (Adobe Photoshop), interpretations.
Literature
    recommended literature
  • MANDÁKOVÁ, Terezie and Martin LYSÁK. Chromosome Preparation for Cytogenetic Analyses in Arabidopsis. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 1, No 1, p. 43-51. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20009. URL info
  • MANDÁKOVÁ, Terezie and Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 4, No 2, p. 359-371. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20022. URL info
  • SCHWARZACHER, T. and HESLOP-HARRISON. Practical in situ hybridization. Bios, 2000. info
Teaching methods
Practical tasks in a cytogenetic laboratory.
Assessment methods
Colloquium.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Note related to how often the course is taught: blok 5 dni.
The course is also listed under the following terms Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C9035