Bi5130 Introduction to human aDNA studies

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. et Mgr. Lenka Falk, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Eva Drozdová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. et Mgr. Lenka Falk, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 11:00–12:50 B11/305
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand the issues of aDNA studies. Students should be able to explain the methods used in aDNA studies and its application in anthropology and archaeology.
Learning outcomes
Students completing the course will be able to: - describe the principles of post-mortem DNA degradation - list the conditions that allowed DNA post mortem preservation and ancient DNA (aDNA)conservation - choose the most suitable part of skeleton for the aDNA analysis - optimize the isolation protocol to get the highest yields of aDNA - edit the PCR protocol due to the complications associated with the character of aDNA - summarize the main importance of aDNA analysis for human evolution and human history research
Syllabus
  • Content of course: general terms, history of aDNA studies
  • sources of human aDNA, issues of aDNA studies
  • sources of contamination, authenticity of results
  • mitochondrial aDNA
  • preparation of samples, decontamination, decalcification
  • extraction of aDNA, PCR
  • electrophoresis, sequencing
  • sex determination
  • identification, kinship analysis
  • evolution studies, disease diagnosis, blood groups.
Literature
    recommended literature
  • HUMMEL, Susanne. Ancient DNA typing : methods, strategies and applications. Berlin: Springer, 2003, xii, 298. ISBN 3540430377. info
  • Ancient DNA : recovery and analysis of genetic material from paleontological, archaeological, museum, medical, and forensic specimens. Edited by Bernd Herrmann - Susanne Hummel. New York: Springer-Verlag, 1994, xi, 263. ISBN 0387943080. info
Teaching methods
lectures
Assessment methods
written test
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/Bi5130