B6400 Methods of molecular biology

Faculty of Science
Spring 2002
Extent and Intensity
3/0/0. 4 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
  • Alberts, Bray, Johnson, Lewis, Raff, Roberts, Walter. Základy buněčné biologie. Espero Publishing. 1998.
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/B6400.htm
The course is also listed under the following terms Spring 2000, Spring 2001.
  • Enrolment Statistics (recent)
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