PřF:C8022 Separation Methods - Course Information
C8022 Separation Methods
Faculty of ScienceSpring 2002
- Extent and Intensity
- 2/0/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
doc. RNDr. Karel Šlais, DrSc. (lecturer) - Guaranteed by
- doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Chemistry Section – Faculty of Science - Prerequisites
- C3100 + C4050 Analytical chemistry (at least C1660) C7021 Separation methods
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, M-CH)
- Analytical Chemistry (programme PřF, N-CH)
- Inorganic Chemistry (programme PřF, M-CH)
- Biochemistry (programme PřF, M-CH)
- Physical Chemistry (programme PřF, M-CH)
- Chemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, M-CH)
- Macromolecular Chemistry (programme PřF, M-CH)
- Organic Chemistry (programme PřF, M-CH)
- Course objectives
- Liquid chromatography (LC), theory and praxis. Separation of macromoleculs (gel permeation chromatography (GPC), light scattering, hydrodynamic chromatography (HDC), field-flow frakcionation (FFF)) + elektroforetic methods = capillary zone electrophoresis (CZE), izotachophoresis (ITP), micelat electrokinetic chromatography (MEKC), gel capillary electrophoresis (GCE)
- Syllabus
- Liquid-liquid extraction. Organic molecules extraction, influence of coexisting phases on extraction, the relationship between analyte structure and its partition coefficient. Extraction of chelates, extraction of ion associates, extraction solvents, influence of pH, synergism, applications of analytical reactions. Extraction flow analysis (FIA), multiple extraction systems, continual extractions, chromatography. Principle of chromatographic separatrion, relation to extraction, theoretical plate, resolution, efficiency, selectivity and retention. Basics of separation optimization, High pressure liquid chromatography (HPLC). Classification of basic separation mechanisms in liquid chromatography (liquid-liquid chromatography, LLC, liquid-solid chromatography, LSC, ion chromatography, IC, gel permeation chromatography, GPC) and their possible combination (ion exclusion in IC, adsorption in GPC etc.) Criteria of selection and optimalization of mobile phases, LC column packing, reverse phase liquid chromatography. Instrumentation of liquid chromatography, pumps, injectors, detectors (diode array detectors, DAD), fluorescence, amperometric, conductivity, refrectometric detectors, miniaturization in LC, applications. Separation of macromolecules Macromolecules: synthetic and biological. Radius of gyration. Relative molecular mass, polydispersity. Optical detection, viscosimetry. Ultracentrifugation, gel capillary electrophoresis. Matrix assisted laser desorption ionization-time of flight (MALDI-TOF). Separation methods for macromolecules: Gel permeation chromatography (GPC), Hydrodynamic chromatography (HDC), Field-flow fractionation (FFF). Special detection techniques. Static ligth scattering (LS), Low-angle laser ligth scattering (LALLS), multi-angle laser light scattering (MALLS). Hyphenation of separation and detection techniques. Electrophoretic methods Types of molecules that could be analyzed by capillary electrophoresis. Proteins. Polypeptides charge. Electrophoretic titration curve. Physical principles of capillary electrophoresis - electrophoretical mobility. Electroosmotic flow - electroosmotic pump, electroosmotic flow modificators - surfactants. Techniques of capillary electrophoresis: capillary zone electrohoresis (free solution capillary electrophoresis), capillary izotachophoresis (CITP), Capillary Gel Electrophoresis, Micellar electrokinetic chromatography, capillary electrochromatography, capillary izoelectric focusing, discontinual electrophoresis. Basics of instrumentation - experimental parametrs: capillary, temeprature control, detectors (indirect detection), sample introduction (injection), reduction of band broadening. Optimization: hea dissipation, electroosmotic flow, oncaillary detection, molecular diffusion, band broadening due to injection. Evaluation of electrophoretic efficiency, advantages od capillary electrophoresis. Evaluation of electropherograms: qualitative and quantitative. Limit of detection. Validation of methods of CE. SDS - Page electrophoresis, capillary ion electrophoresis (CIE), indirect detection, chiral separation, capillary electrohoresis in nonaqueous solvents, DNA analysis, laser induced fluorescence in capillary electrophoresis, capillary electrophoresis - mass spectrometry
- Literature
- CHURÁČEK, Jaroslav and Pavel JANDERA. Separace látek : kapalinová vysokoúčinná kolonová chromatografie. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1981, 140 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POPL, Milan and Jaroslav KUBÁT. Separace látek. 3. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1986, 171 s. info
- VESPALEC, Radim. Chirální separace v kapalném prostředí. 1995, 1 svazek. info
- Assessment methods (in Czech)
- přednáška Zk
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Teacher's information
- http://www.chemi.muni.cz/~pazourek/student/sepmetody.html
- Enrolment Statistics (Spring 2002, recent)
- Permalink: https://is.muni.cz/course/sci/spring2002/C8022