Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2004
Extent and Intensity
1/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
Course objectives
Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
Syllabus
  • Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2004, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2004/Bi8202