PřF:C6790 Mass Spectrometry - Course Information
C6790 Mass Spectrometry
Faculty of ScienceSpring 2006
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Pavel Brož, Ph.D. (lecturer)
prof. RNDr. Jan Vřešťál, DrSc. (lecturer) - Guaranteed by
- prof. RNDr. Jan Vřešťál, DrSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Prerequisites
- Physical Chemistry I. and II., Chemical structure, Quantum chemistry I. (C3140,C4020, C5020, C4060)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, D-CH) (2)
- Analytical Chemistry (programme PřF, M-CH)
- Inorganic Chemistry (programme PřF, D-CH) (2)
- Inorganic Chemistry (programme PřF, M-CH)
- Inorganic Chemistry (programme PřF, N-CH)
- Biochemistry (programme PřF, M-CH)
- Physical Chemistry (programme PřF, D-CH) (2)
- Physical Chemistry (programme PřF, M-CH)
- Physical Chemistry (programme PřF, N-CH)
- Macromolecular Chemistry (programme PřF, D-CH) (2)
- Chemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, D-CH) (2)
- Environmental Chemistry (programme PřF, M-CH)
- Macromolecular Chemistry (programme PřF, M-CH)
- Organic Chemistry (programme PřF, D-CH) (2)
- Organic Chemistry (programme PřF, M-CH)
- Upper Secondary School Teacher Training in Chemistry (programme PřF, M-CH)
- Upper Secondary School Teacher Training in Chemistry (programme PřF, M-SS)
- Course objectives
- Course deals with following chapters: Principles and development of mass spectrometry. Ionisation and desorption methods in mass spectrometry: Electron impact ionisation. Methods of chemical ionisation, field ionisation and desorption. Laser ionisation, MALDI. Fast atom bombardment and secondary ion ionisation. Principles of separation of ions: Magnetic field mass spectrometers. Metastable ions detection. Dynamic mass analysers. Coupling of chromatographic methods with mass spectrometry: GC-MS, LC-MS, thermospray, electrospray. Surface analysis of solids - SIMS. Trace analysis - SSMS, ICP-MS. Direct inlet probe. Membrane introduction mass spectrometry. High temperature mass spectrometry. Search of spectra in mass spectra libraries. The aim of course is to present basic knowledge about mass spectrometry, which enable students to solve practical chemical problems.
- Syllabus
- 1. Mass spectrometry as spectroscopic method. Physico-chemical and analytical information in mass spectrometry. Base peak and molecular peak. 2. Electron ionisation. Ion source, critical potentials, fragmentation. Statistical theory of fragmentation. Field ionisation. 3. Main types of reactions of monomolecular decay of ions of organic compounds. Cleavage of bonds. Rearangements. 4. Methods of chemical ionisation (CI a NCI). Ionisation at atmospheric pressure (API a APCI). Fragmentation of "quasimolecular" ions. Kondensation reaction. 5. Methods of desorption: electric field, laser, plasma 252Cf, fast atoms and ions. 6. Mass analysers I. Introduction to vacuum technique. Sector mass spectrometers. Double focusing instruments. Detection of metastable ions. 7. Mass analysers II. Dynamic analysers. Quadrupole mass spectrometer. Monopole analyser. Ion trap. Ion cyclotron resonance. Time-of-flight mass spectrometer. Detectors of ionts. 8. Coupling of chromatographic methods with mass spectrometry I. Gas chromatography - GC/MS, SFC/MS, TLC/MS. 9. Coupling of chromatographic methods with mass spectrometry II. Liquid chromatography - LC/MS: thermospray, electrospray, particle beam. 10.Tandem mass spectrometry. Collision activation. Arrangement of sector mass spectrometers. Ion trap as tandem. Interpretation of mass spectra. 11.Quantitative mass spectrometry of organic compounds. Types of spectra. Isotopic peaks. Dilution analysis. 12.Mass spectrometry in inorganic chemistry. Analysis of surfaces of solids - SIMS. Trace analysis - SSMS, ICP-MS. 13.High temperature mass spectrometry. Analysis of equilibrium vapour pressures. Thermodynamic data from mass spectrometry. Thermal desorption mass spectrometry (DIP). 14.Special mass spectrometries: membrane introduction (MIMS), electrochemical introduction (DEMS). Good laboratory praxis. Mass spectra libraries.Present state of mass spectrometric instrumentation.
- Literature
- BARKER, James. Mass spectrometry : analytical chemistry by open learning. Edited by David J. Ando. 2nd ed. Chichester: John Wiley & Sons, 1998, xxii, 509. ISBN 0-471-96764-5. info
- Assessment methods (in Czech)
- Výuka probíhá týdně, ukončení je ústní zkouškou. Součástí výuky jsou exkurse k zařízení GC-MS a praktické analýzy hmotnostních spekter.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Spring 2006, recent)
- Permalink: https://is.muni.cz/course/sci/spring2006/C6790