Bi8090 Gene engineering

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 11:00–12:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology, and it is recommended to go through the lectures from Molecular Biology of prokaryotes, Molecular Biology of viruses, Molecular biology of eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and practical application
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods
oral exam
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/Bi8090