C7070 Bioanalytics II - Analytical methods in clinical praxis

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
prof. Ing. Vratislav Chromý, CSc. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 10:00–11:50 AK1
Prerequisites
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are prerequisited.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
main objectives of lecture: introduction into laboratory diagnostics; defining relation between bioanalysis and illness diagnosis; acquainting respective approaches through method selection, analyte determination to results presentation; introduction of basic methodical approaches and common instrumental techniques.
Syllabus
  • 1. introduction, samples - preanalytical phase
  • 2. instrumentation - analysers; organisation, integration and miniaturisation of analyses
  • 3. quality in clinical analysis - check and control
  • 4. analytical methods - selection and optimisation
  • 5. analytical sets; analytical result - its presentation; selected instrumental methods
  • 6. colourness - physical phenomenon and its utilisation in bioanalysis; medical microbiology
  • 7. clinical immunoanalysis
  • 8. clinical aspects of protein analysis
  • 9. clinical aspects of DNA analysis
  • 10. case study - development of a method for clinical diagnostics; role of buffers in bioanalysis
  • 11. selected analytes 1 - inorganic
  • 12. selected analytes 2 - organic and macromolecules
Literature
  • CHROMÝ, Vratislav. Bioanalytika : analytická chemie v laboratorní medicíně. 1. vyd. Brno: Masarykova univerzita v Brně, 2002, 267 s. ISBN 80-210-2917-X. info
  • CHROMÝ, Vratislav and Jiří FISCHER. Analytické metody v klinické chemii. 1. vyd. Brno: Masarykova univerzita v Brně, 2000, 215 s. ISBN 8021023635. info
Assessment methods
the lecture is based on ppt presentation and its explication, presentation its-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which extends the presentation and because there are no available textbooks covering certain parts of the subject.
oral examination; students are required to understand and be familiar with the principles and its applications; enumerations, listing of facts and suchs would not be required.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008.
  • Enrolment Statistics (recent)
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