PřF:C9095 RNA metabolism - Course Information

C9095 RNA metabolism

Extent and Intensity

2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

prof. Mgr. Štěpánka Vaňáčová, Ph.D. (lecturer)

Guaranteed by

prof. Mgr. Štěpánka Vaňáčová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Biochemistry (programme PřF, N-BCH)
• Biophysics (Eng.) (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
• Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
• Biochemistry (Eng.) (programme PřF, D-BCH4)
• Biochemistry (Eng.) (programme PřF, N-BCH)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Biochemistry (programme PřF, N-CH)
• Biomolecular Chemistry (Eng.) (programme PřF, D-BCH4)
• Biomolecular Chemistry (Eng.) (programme PřF, N-BCH)
• Biomolecular Chemistry (programme PřF, D-BCH4)
• Biomolecular Chemistry (programme PřF, N-BCH)
• Ecotoxicology (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, N-BI, specialization Genomika a proteomika)
• Molecular Biology and Genetics (programme PřF, N-BI, specialization Molekularni a geneticka diagnostika)
• Upper Secondary School Teacher Training in Chemistry (programme PřF, N-CH)
• Upper Secondary School Teacher Training in Chemistry (programme PřF, N-SS)

Course objectives

This an advanced molecular biology lecture course focused on RNA structure, function and metablism. Students will learn about different types of RNAs, how they are made and discarded, what is their biological role. The course covers the most recent findings in the RNA field, most of which hav not made it to the molecular biology textbooks yet. Students will also learn the theory of some of the most important methods used to study RNA.

Syllabus

• 1. Introduction to the RNA world 2. Gene expression: prokaryotes versus eukaryotes 3. Transcription of mRNAs 4. mRNA processing: 5' end capping, polyadenylation 5. pre-mRNA splicing 6. tRNAs: transcription, processing, translation 7. rRNAs: transcription, processing, localization, ribosome synthesis 8. noncoding RNAs: snRNAs, snoRNAs 9. noncoding RNAs: miRNAs, piRNAs, siRNAs, dsRNAs 10. RNA silencing 11. RNA surveillance and degradation in the nucleus 12. RNA surveillance and degradation in the cytoplasm 13. Methods to study gene expression: microarray analysis, RT-PCR, Northern blot, 3' extension etc. 14. Methods of analysis of RNA conformation and modification

Literature

• The RNA world : the nature of modern RNA suggests a prebiotic RNA world. Edited by Raymond F. Gesteland - John F. Atkins. New York: Cold Spring Harbor Laboratory Press, 1993, xxiii, 630. ISBN 0-87969-456-4. info

Assessment methods

Lectures, discussions during the lectures, literature presentation. Oral examination.

Language of instruction

English

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

• Enrolment Statistics (Spring 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2009/C9095