PřF:Bi8980 Protein preparation - Course Information

Bi8980 Protein Preparation and Characterization I - Expression and Purification

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

RNDr. Lubomír Janda, Ph.D. (lecturer)
Ing. Blanka Pekárová, Ph.D. (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.

Prerequisites (in Czech)

Bi8202 Proteomics

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-AB)
• Applied Biochemistry (programme PřF, B-AB, specialization Bioanalytical chemistry)
• Applied Biochemistry (programme PřF, B-AB, specialization Biotechnology)
• Applied Biochemistry (programme PřF, B-AB, specialization Clinical biochemistry)
• Biophysics (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
• Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
• Biochemistry (programme PřF, B-BCH)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Ecotoxicology (programme PřF, N-BI)
• Genomics and Proteomics (programme PřF, D-BI4)
• Molecular and Cellular Biology (programme PřF, D-BI4)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)
• General and Molecular Genetics (programme PřF, D-BI4)
• General Biology (programme PřF, N-BI)
• General Biology (programme PřF, N-BI, specialization Ekotoxikologie)
• General Biology (programme PřF, N-BI, specialization Plant Physiology)
• General Biology (programme PřF, N-BI, specialization Physiology of Animals)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

This course is for students to gain the basic knowledge about the preparation of proteins in sufficient quantity and quality for crystallization, NMR analysis and others biochemical measurements. At the end of this course the student will be able to: understand the different approaches in the cloning of various expression vectors using affinity tags or without tags, and subsequent purification of the recombinant protein with affinity chromatography or classically; to remove the fusion protein (anchor); to hold the protein in soluble state; to know various methods for protein detection during the purification procedures.

Syllabus

• 1. The molecular principles for understanding proteins. 2. Calculation in the molecular biosciences 3. The important properties of proteins and how to explore them. 4. DNA cloning. 5. Protein expression. 6. Cell disruption. 7. Fusion proteins and affinity purification. 8. Protein purification chromatography. 9. Protein quality. 10 Analytical methods used to study proteins. 11. Protein labelling. 12. Invited lecture.

Teaching methods

Lectures

Assessment methods

written test + oral exam

Language of instruction

Czech

Follow-Up Courses

• Bi8858 Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology
• C8857 Protein Preparation and Characterization III - Protein-mediated interactions

Further Comments

Study Materials
The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (Spring 2010, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2010/Bi8980