PřF:Bi8980c Protein preparation-practice - Course Information

Bi8980c Protein Preparation and Characterization I - practice

Extent and Intensity

0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).

Teacher(s)

RNDr. Lubomír Janda, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.

Prerequisites (in Czech)

Bi8980 Protein preparation

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-AB)
• Applied Biochemistry (programme PřF, B-AB, specialization Bioanalytical chemistry)
• Applied Biochemistry (programme PřF, B-AB, specialization Biotechnology)
• Applied Biochemistry (programme PřF, B-AB, specialization Clinical biochemistry)
• Biophysics (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
• Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
• Biochemistry (programme PřF, B-BCH)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Genomics and Proteomics (programme PřF, D-BI4)
• Molecular and Cellular Biology (programme PřF, D-BI4)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)
• General and Molecular Genetics (programme PřF, D-BI4)
• General Biology (programme PřF, N-BI)
• General Biology (programme PřF, N-BI, specialization Ekotoxikologie)
• General Biology (programme PřF, N-BI, specialization Plant Physiology)
• General Biology (programme PřF, N-BI, specialization Physiology of Animals)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

The practical course intended for students in the early stages of their research career, will deal overexpression of proteins in prokaryotic systems with different expression vectors, strategy of protein purification and their characterisation. At the end of this practical course the student will be able to: use the bioinformatics in analysis and planning of a protein expression project; design primers and optimize gene expression; compare in vectors containing different tags and fusion proteins and different strains; optimize the quality of your favorite protein during different purification strategies.

Syllabus

• 1.Strategy of cloning into expression plasmids. 2. Expression and purification of the human scFv produced in cytoplasm or periplasmic space. 3. Expression and purification of the plant protein (A. pET32 – thioredoxin - chaperon/B. pRSET – His.tag/ C. pET161DEST – Gate way technology – Lumio.tag/D. pET28 – without any tag.)

Teaching methods

Practical course

Assessment methods

protocol, written test

Language of instruction

Czech

Follow-Up Courses

• Bi8858c Protein Preparation and Characterization II - practice
• C8857c Protein Preparation and Characterization III - practice

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: blok 5 dnů.

• Enrolment Statistics (Spring 2010, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2010/Bi8980c