Bi4090c General microbiology - practical course

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor)
RNDr. Kateřina Kšicová (seminar tutor)
Guaranteed by
RNDr. Ludmila Tvrzová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of this course, students should be able to work aseptically, to inoculate and cultivate bacteria, to prepare stained and native microscopic slides, to determine number of bacteria in different samples, to determine indicators of microbial water quality, to use some basic identification methods.
Syllabus
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Determination of the number of phage particles in lysate of bacterial cells 10. Yeasts, test of vitality 11. Microorganisms in soil. Inoculation of microscopic fungi. 12. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 13. Microscopic fungi. 14. Reports, test and microscopic examination/determination of unknown bacterial strain.
Literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical classes, written reports, written test, credit
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.