Bi8202c Proteomics - practice

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.