C9095 RNA metabolism

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
prof. Mgr. Štěpánka Vaňáčová, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Štěpánka Vaňáčová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should have knowledge about different types of RNAs, how they are made and discarded, what is their biological role. The course covers the most recent findings in the RNA field, most of which hav not made it to the molecular biology textbooks yet. Students will also learn the theory of some of the most important methods used to study RNA.
Syllabus
  • 1. Introduction to the RNA world 2. Gene expression: prokaryotes versus eukaryotes 3. Transcription of mRNAs 4. mRNA processing: 5' end capping, polyadenylation 5. pre-mRNA splicing 6. tRNAs: transcription, processing, translation 7. rRNAs: transcription, processing, localization, ribosome synthesis 8. noncoding RNAs: snRNAs, snoRNAs 9. noncoding RNAs: miRNAs, piRNAs, siRNAs, dsRNAs 10. RNA silencing 11. RNA surveillance and degradation in the nucleus 12. RNA surveillance and degradation in the cytoplasm 13. Methods to study gene expression: microarray analysis, RT-PCR, Northern blot, 3' extension etc. 14. Methods of analysis of RNA conformation and modification
Literature
  • The RNA world : the nature of modern RNA suggests a prebiotic RNA world. Edited by Raymond F. Gesteland - John F. Atkins. New York: Cold Spring Harbor Laboratory Press, 1993, xxiii, 630. ISBN 0-87969-456-4. info
Teaching methods
This class is taught in the form of theory lectures. In each lecture, students are asked to solve a task related to the particular theme.
Assessment methods
Lectures, discussions during the lectures, literature presentation. Oral examination.
Language of instruction
English
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every other week.
The course is also listed under the following terms Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Autumn 2023.