PřF:C9085 Protein-RNA interactions - Course Information

C9085 Protein-RNA interactions

Extent and Intensity

1/0/0. 1 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).

Teacher(s)

prof. Mgr. Richard Štefl, Ph.D. (lecturer)

Guaranteed by

prof. Mgr. Richard Štefl, Ph.D.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 14:00–15:00 C04/211

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Biochemistry (programme PřF, N-BCH)
• Biophysics (Eng.) (programme PřF, D-FY)
• Biophysics (Eng.) (programme PřF, N-FY)
• Biophysics (programme PřF, D-FY)
• Biophysics (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
• Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
• Biochemistry (Eng.) (programme PřF, D-BCH)
• Biochemistry (Eng.) (programme PřF, N-BCH)
• Biochemistry (Eng.) (programme PřF, N-BCH3)
• Biochemistry (programme PřF, D-BCH)
• Biochemistry (programme PřF, N-BCH)
• Biochemistry (programme PřF, N-BCH3)
• Biochemistry (programme PřF, N-CH)
• Biomolecular Chemistry (Eng.) (programme PřF, D-BCH)
• Biomolecular Chemistry (Eng.) (programme PřF, N-BCH)
• Biomolecular Chemistry (Eng.) (programme PřF, N-BCH3)
• Biomolecular Chemistry (programme PřF, D-BCH)
• Biomolecular Chemistry (programme PřF, N-BCH)
• Biomolecular Chemistry (programme PřF, N-BCH3)
• Ecotoxicology (programme PřF, N-BI)
• Microbiology (Eng.) (programme PřF, D-BI)
• Microbiology (programme PřF, D-BI)
• Molecular and Cellular Biology (Eng.) (programme PřF, D-BI)
• Molecular and Cellular Biology (programme PřF, D-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, N-BI, specialization Genomika a proteomika)
• Molecular Biology and Genetics (programme PřF, N-BI, specialization Molekularni a geneticka diagnostika)
• General and Molecular Genetics (Eng.) (programme PřF, D-BI)
• General and Molecular Genetics (programme PřF, D-BI)
• Upper Secondary School Teacher Training in Chemistry (programme PřF, N-CH)
• Upper Secondary School Teacher Training in Chemistry (programme PřF, N-SS)

Course objectives

At the end of the course students should be able to understand and explain how proteins interact with RNAs. Different modes of interactions as well as their importance for biological functions will be discussed. In addition, the course will highlight advantages and disadvantages of various techniques when applied to protein-RNA complexes.

Syllabus

• 1. A Brief introduction to chemical structure of RNAs and proteins 2. A Brief introduction to basic structural motifs of RNAs and proteins 3. Introduction to protein-RNA interactions 4. RNA sequence-dependent recognition by proteins 5. RNA structure-dependent recognition by proteins 6. Experimental methods for structural determination of protein-RNA complexes 7. Biochemical methods for characterization of protein-RNA complexes 8. Known mechanistic pictures on how the interactions between Proteins and RNAs mediate gene expression and its regulation.

Literature

• VOET, Donald and Judith G. VOET. Biochemistry. 3rd ed. Hoboken: John Wiley & Sons, 2004, xv, 1591. ISBN 0471392235. info

Teaching methods

lectures & class discussions

Assessment methods

lectures, class discussion, oral exam

Language of instruction

English

Further Comments

Study Materials
The course is taught each semester.

• Enrolment Statistics (Spring 2011, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2011/C9085