Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.