Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2012
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/235
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (recent)
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