C7943 Bioanalytics II - Laboratory medicine

Přírodovědecká fakulta
jaro 2014
Rozsah
2/0/0. 2 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučující
doc. Mgr. Jan Havliš, Dr. (přednášející)
Garance
doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Rozvrh
Čt 13:00–14:50 C02/211
Předpoklady
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are pre-requisited.
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Cíle předmětu
main objectives of lecture: introduction into laboratory diagnostics; at the end of the course students should be able - to define relation between bioanalysis and illness diagnosis; - to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation; - to undestand principles of basic methodical approaches and common instrumental techniques.
Osnova
  • 1. introduction, samples - preanalytical phase
  • 2. instrumentation - analysers; organisation, integration and miniaturisation of analyses
  • 3. quality in clinical analysis - check and control
  • 4. analytical methods - selection and optimisation
  • 5. analytical sets; analytical result - its presentation; selected instrumental methods
  • 6. colourness - physical phenomenon and its utilisation in bioanalysis; medical microbiology
  • 7. clinical immunoanalysis
  • 8. clinical aspects of protein analysis
  • 9. clinical aspects of DNA analysis
  • 10. case study - development of a method for clinical diagnostics; role of buffers in bioanalysis
  • 11. selected analytes 1 - inorganic
  • 12. selected analytes 2 - organic and macromolecules
Literatura
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis: Saunders/Elsevier, 2008, xx, 952. ISBN 9780721638652. info
  • Clinical laboratory diagnostics : use and assessment of clinical laboratory results. Edited by Lothar Thomas. 1st ed. Frankfurt am Main: TH-Books, 1998, 1527 s. ISBN 3980521540. info
Výukové metody
the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
Metody hodnocení
oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Předmět je zařazen také v obdobích jaro 2013, jaro 2015, jaro 2016, jaro 2017, jaro 2018, jaro 2019, jaro 2020, jaro 2021, jaro 2022, jaro 2023, jaro 2024, jaro 2025.