C7943 Bioanalytics II - Laboratory medicine

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Thu 13:00–14:50 C02/211
Prerequisites
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are pre-requisited.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
main objectives of lecture: introduction into laboratory diagnostics; at the end of the course students should be able - to define relation between bioanalysis and illness diagnosis; - to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation; - to understand principles of basic methodical approaches and common instrumental techniques.
Syllabus
  • 1. introduction, samples - preanalytical phase
  • 2. instrumentation - analysers; organisation, integration and miniaturisation of analyses
  • 3. quality in clinical analysis - check and control
  • 4. analytical methods - selection and optimisation
  • 5. analytical sets; analytical result - its presentation; selected instrumental methods
  • 6. colourness - physical phenomenon and its utilisation in bioanalysis; medical microbiology
  • 7. clinical immunoanalysis
  • 8. clinical aspects of protein analysis
  • 9. clinical aspects of DNA analysis
  • 10. case study - development of a method for clinical diagnostics; role of buffers in bioanalysis
  • 11. selected analytes 1 - inorganic
  • 12. selected analytes 2 - organic and macromolecules
Literature
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis: Saunders/Elsevier, 2008, xx, 952. ISBN 9780721638652. info
  • Clinical laboratory diagnostics : use and assessment of clinical laboratory results. Edited by Lothar Thomas. 1st ed. Frankfurt am Main: TH-Books, 1998, 1527 s. ISBN 3980521540. info
Teaching methods
the lecture is based on ppt presentation and its explication. presentation itself will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in English covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and to be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Language of instruction
English
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2014, recent)
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