C9095 RNA metabolism

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
prof. Mgr. Štěpánka Vaňáčová, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Štěpánka Vaňáčová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Upon successful completion of the course, students will be able: to identify and describe the major classes of RNAs in Eukaryotic cells, to compare their primary, secondary and tertiary structures, to demonstrate the differences in the transcription, processing and function between different types of coding and noncoding RNAs. The students will be able to design an experimental approach how to study the function of given RNA. Moreover, the students will be able to assess the importance of RNA biology studies in mammalian development and disease.
Syllabus
  • 1. Introduction to the RNA world 2. Gene expression: prokaryotes versus eukaryotes 3. Transcription of mRNAs 4. Messenger RNA processing: 5' end capping, polyadenylation 5. pre-mRNA splicing 6. RNA editing and trans-splicing 7. Ribosomal RNAs: transcription, processing, localization, ribosome synthesis 8. Noncoding RNAs I: snRNAs, snoRNAs, tRNAs 9. Noncoding RNAs II: CUTs, XUTs, SUTs, PROMPTs etc. 10. RNA silencing 11. RNA surveillance and degradation in the nucleus 12. Translation of mRNA 13. Methods to study gene expression: microarray analysis, RT-PCR, Northern blot, 3' extension, EMSA, RIP-Seq, ChIP-Seq etc.
Literature
    recommended literature
  • RNA Biology. Gunter Meister, Wiley-VCH, Weinheim 2011, XVI+363 pp., ISBN 978-3-527-32278-7
Teaching methods
This class is in the form of lectures and class discussions.
Assessment methods
Ten written tests during the lecture courses followed by oral examination. Each test consists of two questions from the preceding lecture. Students can use their notes to answer the questions. 100% of correct answers is needed to pass with the grade A without following oral examination. If less than 100% answers correct, students are given a chance to correct the answers during final oral examination. 50% correct answers are required to pass the exam.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednáška se koná, pokud počet zapsaných studentů je vyšší než 5.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Autumn 2023.
  • Enrolment Statistics (Spring 2016, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2016/C9095