Bi4020c Essential methods in molecular biology

Faculty of Science
Spring 2019
Extent and Intensity
0/1/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Thu 11:00–11:50 B11/132
Prerequisites
( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )) && ! Bi4010 Essential molecular biology && ! Bi6400 Methods of molecular biology
Basic knowledge of general genetics, physiology, anorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This lecture is conceived as an introduction into molecular biology methods and it is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who are not specialized in molecular biology. Understanding of principle and practical use of molecular biology methods is emphasized.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and make reasoned decisions about choosing the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Structure of nucleic acids - seminar (primary, secondary and tertiary structure of DNA and RNA, different conformations of DNA and their significance in biological systems).
  • 2. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 3. Electrophoretic analyses of nucleic acids.
  • 4. Hybridisation of nucleic acids: probe preparation, labelling.
  • 5. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. DNA cloning (characterization and using of basic types of vectors, cloning strategies, DNA transfer to prokaryotic cells).
  • 8. Polymerase chain reaction.
  • 9. Basic methods of molecular diagnostics.
  • 10. Basic methods of bioinformatics.
Literature
  • BROWN, T. A. Gene cloning and DNA analysis : an introduction. 5th ed. Oxford: Blackwell Publishing, 2006, xx, 386. ISBN 1405111216. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • ROSYPAL, Stanislav. Úvod do molekulární biologie. Třetí inovované vydání. Brno, 1998, 300 pp. Díl I. Informační makromolekuly. ISBN 80-902562-0-1. URL info
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination consists of written test containing 30 questions.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2019/Bi4020c