C9085 Protein-RNA interactions

Faculty of Science
Spring 2020
Extent and Intensity
1/0/0. 1 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
prof. Mgr. Richard Štefl, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Richard Štefl, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Fri 9:00–9:50 C04/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course will provide an overview on how proteins interact with RNAs. Different modes of interactions as well as their importance for biological functions will be discussed. In addition, the course will highlight advantages and disadvantages of various techniques when applied to protein-RNA complexes.
Learning outcomes
At the end of the course students should be able to understand and explain how proteins interact with RNAs. Different modes of interactions as well as their importance for biological functions will be discussed. In addition, the course will highlight advantages and disadvantages of various techniques when applied to protein-RNA complexes.
Syllabus
  • 1. A Brief introduction to chemical structure of RNAs and proteins 2. A Brief introduction to basic structural motifs of RNAs and proteins 3. Introduction to protein-RNA interactions 4. RNA sequence-dependent recognition by proteins 5. RNA structure-dependent recognition by proteins 6. Experimental methods for structural determination of protein-RNA complexes 7. Biochemical methods for characterization of protein-RNA complexes 8. Known mechanistic pictures on how the interactions between Proteins and RNAs mediate gene expression and its regulation.
Literature
  • VOET, Donald and Judith G. VOET. Biochemistry. 3rd ed. Hoboken: John Wiley & Sons, 2004, xv, 1591. ISBN 0471392235. info
Teaching methods
lectures & class discussions
Assessment methods
lectures, class discussion, oral exam
Language of instruction
English
Further Comments
Study Materials
The course is taught each semester.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2020/C9085