Bi8090 Gene engineering

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Thu 16:00–17:50 online_B1
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2021, recent)
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