PřF:C8980c Protein preparation-practice - Course Information

C8980c Protein Preparation and Characterization I - practice

Extent and Intensity

0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

RNDr. Lubomír Janda, Ph.D. (lecturer)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites

C8980 Protein preparation || NOW( C8980 Protein preparation )
Basic knowledge of biochemistry and/or molecular biology is prerequisite for registration for the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-AB)
• Applied Biochemistry (programme PřF, B-AB, specialization Bioanalytical chemistry)
• Applied Biochemistry (programme PřF, B-AB, specialization Biotechnology)
• Applied Biochemistry (programme PřF, B-AB, specialization Clinical biochemistry)
• Biophysics (programme PřF, N-FY)
• Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
• Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
• Biochemistry (programme PřF, B-BCH)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Molecular and Cellular Biology (programme PřF, D-BI4)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• General and Molecular Genetics (programme PřF, D-BI4)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Ekotoxikologie)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

Students completing the practical course will be able to use expression system for protein production, select suitable purification method and to analyze obtained results.

Learning outcomes

Upon completion of the laboratory course, students are able to: - design a cloning strategy - express protein in a heterologous prokaryotic system - purify protein (protein precipitation, affinity, ion-exchange and size-exclusion chromatography) - analyse protein function and purity.

Syllabus

• 1. Bioinformatics and theory of DNA cloning. 2. Protein expression in bacteria. 3. Cell desintegration. 4. Purification. 5. SDS-PAGE. 6. protein activity measurement.

Literature

recommended literature
• HARDIN, Charles. Cloning, gene expression and protein purification : experimental procedures and process rationale. New York: Oxford University Press, 2001, viii, 435. ISBN 0195132947. info
• Guide to protein purification. Edited by Richard R. Burgess - Murray P. Deutscher. 2nd ed. Boston: Elsevier/Academic Press, 2009, liii, 851. ISBN 9780123749789. info

Teaching methods

Practical course

Assessment methods

Laboratory protocol, results presentation

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: blok 5 dnů.

• Enrolment Statistics (Spring 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2021/C8980c