CG001 Introduction to genomics and proteomics

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Jan Paleček, Dr. rer. nat. (lecturer)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
C3181 Biochemistry I
successful completion of the course requires basic orientation in biochemistry (C3181) and molecular biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
major goal of this subject is to acquaint students with basics in genomics and proteomics as important field of modern biochemistry.
Learning outcomes
at the end of the course students should be able: to understand and to explain fundamental terms related to genomics & proteomics; to know about composition of and differences between genomes of different organisms; to understand different structural levels of genomes; to know about processes taking part at genomes including protein expression; to know relations between protein properties and methods of their study to use this knowledge on proteins and their complexes to follow their role in organisms.
Syllabus
  • genomics (Jan Paleček)
  • I. Introduction to genomics and proteomics
  • Genomes, transcriptomes, proteomes; Genotype and phenotype
  • II. Genome analysis
  • Genetic and physical maps, sequencing; Gene identification, annotation; PCR, cloning; Functional analysis of genes
  • III. Genomes anatomy
  • Genomes of prokaryotes and organelles; Nuclear genomes (eukaryotes); Genomes of viruses and mobile elements
  • IV. How are genomes expressed
  • Transcription factors – transcription – translation; Higher order chromatin structure; Genome in cellular environment
  • V. How do genomes replicate
  • Replication; Mutations, repair and recombination; Evolution
  • VI. Examples of genomic studies
  • proteomics (Jan Havliš)
  • I. proteins
  • what are proteins? how did they come to exist? where did the proteins come from? what are they good for in the cell?
  • II. proteomics proteome is more than just a lot of proteins, a prototype. why is it good to study proteome / proteotype? how is it done?
  • III. protein identification
  • what do we have in the cell for proteins? how do we recognize them and distinguish one from another? proteoforms
  • IV. protein quantification
  • how much of our protein is in the cell? how can it be measured? assay specificity is important
  • V. protein structure
  • what is it good for to know protein structure? how can we determine it? motifs and domains, function predictions.
  • VI. protein interactions
  • how and why do proteins interact with each other? how do we find these interactions? protein-protein interactions, protein complexes.
Teaching methods
the lecture is based on ppt presentations and their explication. presentations themselves will be available as study materials (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks in Czech language covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and to be familiar with the principles and their applications. examination consists of four basic questions (2+2 from genomics & proteomics), which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
videocon lectures will take place in the MS teams environment. if possible, download the environment application. all MU students have access to it via office 365, see "external services" in IS. i recommend learning to work with the application in advance. the use of a browser version restricts your access to some useful functions. the lecture will be recorded in mp4 format and then uploaded in the study materials in IS. MS teams guides: https://is.muni.cz/auth/do/mu/samostudium/pages_en/02-online.html
The course is also listed under the following terms Spring 2022.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2021/CG001