Bi4020c Molecular biology - laboratory practice

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. Mgr. Petr Beneš, Ph.D. (seminar tutor)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Mgr. Jarmila Navrátilová, Ph.D. (seminar tutor)
Mgr. Jiří Kohoutek, Ph.D. (seminar tutor)
Mgr. Lucia Knopfová, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Petr Beneš, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Petr Beneš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi4020c/01_1: Wed 13:00–16:50 D36/216, L. Knopfová, J. Kohoutek
Bi4020c/01_2: Wed 13:00–16:50 D36/216, L. Knopfová, J. Kohoutek
Bi4020c/02_1: Wed 9:00–12:50 D36/216, S. Koudelková, J. Navrátilová
Bi4020c/02_2: Wed 9:00–12:50 D36/216, S. Koudelková, J. Navrátilová
Bi4020c/03: Fri 12:00–15:50 D36/216, S. Koudelková
Bi4020c/04: Thu 13:00–16:50 D36/216, T. Botka
Prerequisites
( C1600 Laboratory in Chemistry || C1080 General Chemistry-lab.course || C1620 General and Inorg.Chem.-lab. || C1650 Fundamentals of Chem.-lab. || C2100 Inorganic Chemistry-lab.course || C2500 General Chemistry-lab.course || C2740 Fundament. of Chem.-lab.course || C2480 Basic Organic Chem. and Bioch. || Bi9410 Structural Biology || SOUHLAS) && ( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )) && !NOWANY( Bi4010c Essential mol. biol - seminar , Bi4035 Molecular biology - lab.pract. ) && ! Bi4010c Essential mol. biol - seminar && ! Bi4035 Molecular biology - lab.pract.
Basic knowledge of structure and function of prokaryotic and eukaryotic genome, aseptic microbiological techniques, and preparation of chemical solutions. Participants should have already passed basic practicals from organic and anorganic chemistry and microbiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 150 student(s).
Current registration and enrolment status: enrolled: 12/150, only registered: 0/150
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to isolate and analyze genetic material including chromosomal and plasmid DNA.
Learning outcomes
Student will be able to:
isolate plasmid DNA of sufficient quality and quantity;
analyze isolated genetic material by basic methods of molecular biology such as restriction endonuclease cleavage and electrophoresis;
prepare sufficient amount of PCR product of the gene of interest;
create the construct of the plasmid vector and the insert of the gene of interest and perform the transformation of competent cells;
use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • This course include the practical tasks to learn the basic methods of molecular biology, including: Preparation of basic chemical solutions Isolation of plasmid DNA Determination of DNA concentration and purity Restriction cleavage Electrophoresis PCR Centrifugation
Literature
Teaching methods
Students perform laboratory experiments according to curriculum. Study materials are available in the MUNI IS.
Assessment methods
Students independently manage the entered tasks and correctly interpret the results in laboratory protocols.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/Bi4020c