C6790 Mass Spectrometry

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Pavel Brož, Ph.D. (lecturer)
prof. RNDr. Jan Vřešťál, DrSc. (lecturer)
Guaranteed by
doc. RNDr. Pavel Brož, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Prerequisites
Physical Chemistry I. and II., Chemical structure, Quantum chemistry I. (C3140,C4020, C5020, C4060)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 28 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with basic information on mass spectrometry and its use for the study of chemical structure and for physicochemical and analytical purposes. The student will get an overview of individual mass spectrometry techniques and ability to analyze mass spectra. This knowledge will allow him to be oriented when using the method in practice.
Learning outcomes
At the end of the course, student will be able to:
- be knowledgeable in the use of mass spectrometry in chemistry
- choose a suitable mass spectrometry technique with respect to the nature of the analyzed substance
- use a mass spectrometer for qualitative and quantitative analysis and for obtaining physicochemical information on chemical substances
- analyze mass spectra, use mass spectral databases and solve chemical structure
Syllabus
  • 1. Mass spectrometry as spectroscopic method. Physico-chemical and analytical information in mass spectrometry. Base peak and molecular peak.
  • 2. Electron ionisation. Ion source, critical potentials, fragmentation. Statistical theory of fragmentation. Field ionisation.
  • 3. Main types of reactions of monomolecular decay of ions of organic compounds. Cleavage of bonds. Rearangements.
  • 4. Methods of chemical ionisation (CI a NCI). Ionisation at atmospheric pressure (API a APCI). Fragmentation of "quasimolecular" ions. Condensation reaction.
  • 5. Methods of desorption: electric field, laser, plasma 252Cf, fast atoms and ions.
  • 6. Mass analysers I. Introduction to vacuum technique. Sector mass spectrometers. Double focusing instruments. Detection of metastable ions.
  • 7. Mass analysers II. Dynamic analysers. Quadrupole mass spectrometer. Monopole analyser. Ion trap. Ion cyclotron resonance. Time-of-flight mass spectrometer. Detectors of ions.
  • 8. Coupling of chromatographic methods with mass spectrometry I. Gas chromatography - GC/MS, SFC/MS, TLC/MS.
  • 9. Coupling of chromatographic methods with mass spectrometry II. Liquid chromatography - LC/MS: thermospray, electrospray, particle beam.
  • 10.Tandem mass spectrometry. Collision activation. Arrangement of sector mass spectrometers. Ion trap as tandem. Interpretation of mass spectra.
  • 11.Quantitative mass spectrometry of organic compounds. Types of spectra. Isotopic peaks. Dilution analysis.
  • 12.Mass spectrometry in inorganic chemistry. Analysis of surfaces of solids - SIMS. Trace analysis - SSMS, ICP-MS.
  • 13.High temperature mass spectrometry. Analysis of equilibrium vapour pressures. Thermodynamic data from mass spectrometry. Thermal desorption mass spectrometry (DIP).
  • 14.Special mass spectrometries: membrane introduction (MIMS), electrochemical introduction (DEMS). Good laboratory praxis. Mass spectra libraries.Present state of mass spectrometry instrumentation.
Literature
  • BARKER, James. Mass spectrometry : analytical chemistry by open learning. Edited by David J. Ando. 2nd ed. Chichester: John Wiley & Sons, 1998, xxii, 509. ISBN 0-471-96764-5. info
Teaching methods
Theoretical preparation in lectures containing many practical examples. Control questions on the subject taught will continuously be given the students for working out.
Assessment methods
Oral examination and analysis of 2 mass spectra. The worked out control questions on the subject taught will also be the part of the assessment.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/C6790