C7830 Capillary Electrophoresis

Faculty of Science
Spring 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Josef Havel, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Viktor Kanický, DrSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Wed 8:00–9:50 C14/207
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Separation methods play significant role not only in analytical chemistry but also in all other chemistry branches, in chemical and biochemical technology and pharmaceutical industry. Capillary electrophoresis is modern separation method and one of the most efficient separation techniques, generally. The main advantage besides its high efficiency is low consumption of the sample and possibility to separate even very similar compounds and various isomers including the chiral ones. The main goal of the course is to explain the students the basic principles in such way that they would be able on the base of gained knowledge to use rationally the technique in real practice and to know how to apply it even to the separation problems not described in the literature: for separation and determination of inorganic, organic, pharmaceutics and drugs and also to use it in other branches like biology, medicine and technology. Therefore, part of the course is also practical exercise and problem solving on a computer and simulation programs afforded with extensive database of compounds, theirs physico-chemical constants, etc. which allows to learn the students to think. The course is also accompanied with basics of the electrophoretic methods development and optimization including the use of a combination of the experimental design and with the use of artificial neural networks.
Learning outcomes
Student after passing the subject should be able: - understand principals of individual modes of capillary electrophoresis (CE);
- should be able to explain basic principals of CE also to friends and other students;
- propose the suitable method of separation for given analytes and explain why;
- to understand basic principals of detection in CE and should be able to recommend suitable detection method for given class of compounds;
- especially, he should understand basic principal of in-direct detection in CE and propose for given type of analytes suitable method of indirect detection;
- to understand databases of compounds properties in the literature(pK, mobilities, etc.) and be able to use the data I n the proposal of separation of given compounds by ;
Syllabus
  • History of development. Basics of electromigration Placement of Capillary electrophoresis in separation methods Basic modes of electrophoresis Basic terms: ionic mobility, effective mobility Calculation of ionic and effective mobility, determination Electroosmotic flow (EOF), principles. Stern double layer; Zeta potential Possibilities how to measure EOF Modification of EOF with various approaches Principles of the instrumentation, scheme of the instrument, capillary dimensions, detection windows Technical details; uncoated and coated capillaries Detectors and detection techniques in CE Uv Vis, DAD, potentiometric, conductivity, mass spectrometry and laser induced fluorescence Hyphenation of CE and MS ev. CE and MALDI Methods of sample injection in CE; hydrodynamic and electrokinetics; differences. Basic electrolyte; pH changes, various buffers Dispersion, main factors Ohm law, effect of temperature, elimination-cooling Electrokinetic micellar chromatography, principals and separation of neutral compounds Chemical equilibria and kinetics in CE Effect of chemical reactions on the separation;the role of complexation and its use Chiral separation, chiral selectors, possibilities of the influence to chiral separation Various applications of CE ; brief survey Optimization in CE; basic possibilities; Single-factor experiments; multivariate approaches and artificial neural networks Possibilities of next development; chip electrophoresis.
Literature
  • JANDIK, Petr and Günther BONN. Capillary electrophoresis of small molecules and ions. New York: VCH Publishers, 1993, 298 s. ISBN 1-56081-533-7. info
  • KUHN, Reinhard and Sabrina HOFFSTETTER-KUHN. Capillary electrophoresis : principles and practice. Berlin: Springer-Verlag, 1993, x, 375 s. ISBN 3-540-56434-9-. info
  • CHANKVETADZE, Bezhan. Capillary electrophoresis in chiral analysis. Chichester: John Wiley & Sons, 1997, xiii, 555. ISBN 0471974153. info
  • Affinity capillary electrophoresis in pharmaceutics and biopharmaceutics. Edited by Reinhard Neubert - Hans-Hermann Rüttinger. New York: Marcel Dekker, 2003, xi, 362. ISBN 0824709519. info
  • KUHN, Reinhard and Sabrina HOFFSTETTER-KUHN. Capillary electrophoresis : principles and practice. Berlin: Springer-Verlag, 1993, x, 375. ISBN 0387564349. info
  • SCHWARTZ, Herb. Separation of proteins and peptides by Capillary Electrophoresis: Application to Analytical Biotechnology. Beckman Coulter, 1993, 119 pp. info
  • Capillary electrophoresis in analytical biotechnology. Edited by Pier Giorgio Righetti. Boca Raton: CRC Press, 1996, 551 s. ISBN 0-8493-7825-7. info
  • LI, Sam F. Y. Capillary electrophoresis : principles, practice and applications. Amsterdam: Elsevier, 1993, 582 s. ISBN 0444815902. info
  • Capillary electrophoresis of proteins and peptides. Edited by Mark A. Strege - Avinash L. Lagu. Totowa, N.J.: Humana Press, 2004, xi, 332. ISBN 1588290174. info
  • Capillary electrophoresis of nucleic acids. Edited by Keith R. Mitchelson - Jing Cheng. Totowa, N.J.: Humana Press, 2001, xx, 408. ISBN 0896037657. info
Teaching methods
Lectures and demonstration of separation processes and exercises on a computer.
Assessment methods
Written test combined with oral examination
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2025.
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