GENOTYP FENOTYP >chsl atgacagaat acaggatgac tatgacgtga cggcttatat gatgacc... >chsl MFVDDHLA VNQNFYLR SHRQL... GEN.KOD STRUKTURA FUNKCE bloinfoivn centr ":^J Levels of protein structure: 0,1 Zeroth: amino acid composition - no structural information Primary ■ This is simply the order of covalent linkages along the polypeptide chain, i.e. the sequence itself MHGAYRTPRSKT AYGCQIL TRAS •c»K27 Levels of protein structure: 2 ■ Secondary ■ Local organization of the protein backbone: a-helix, ß-strand (which assemble into p-sheets), turn and interconnecting loop blotnfqrnvuir Levels of protein structure: 3 Tertiary ■ packing of secondary structure elements into a compact spatial unit ■ "Fold" or domain -this is the level to which structure prediction is currently possible Structural classes: 2 a/ß (parallel p-sheet) a+ß (antiparallel p-sheet) Analýza sekvence proteinů - statistická analýza - identifikace motivů (vzorů) typických pro vybrané funkce - strukturní modelování (ab initio, fragmentové metody, threading, homologní modelování) - další nástroje pro předpovídání funkce a struktury Statistická analýza Zastoupení dipeptidů v protánových sekvencích 1384 MM 1348 HW 1345 PC 24174 LL 18928 SG 1339 QW 1337 NC 18914 SL 1324 WF 18843 AL 1267 CN 18785 LA 1241 CF 18675 AA 18629 LS 17616 SS 1175WQ 1156QC 1138CY 17397 GL 1087 WM 16309 GG 1026 HM 16120 LG 985 WP 15790 AS 929 HC 15769 LE 883 CH 15510 LV 718 WH 15416 AG 718 CC 15390 AV 640 CM 15328 GS 553 MC 15319 VL 526 WW Statistická analýza Zložení dipeptidů lze využít k hodnocení podobnosti, které je určitým způsobem dokonalqší než Needleman-Wjnsch MASAQSFYLLFNMVLADHSHQ MA, AS, SA, AQ, QS, FY, YL, LL, LF, FN, NM, MV, VL, LA, AD, DH, HS, SH, HQ FNMVLADHSHQMASAQSFYLL MA, AS, SA, AQ, QS, FY, YL, LL, HQ, FN, NM, MV, VL, LA, AD, DH, HS, SH, HQ Algoritmus pro globální srovnání pomocí DP ABCNJ RQCLCRPM ABCNJ RQCLCRPM ABCNJ RQCLCRPM A J C J N R C K C R B 1 1 1 11 1 1 1 Ti j 1 hl i 1 1 1 1 1 1 1 2:11111111 0 0 OOOOOOOOOO010 1 1 1 1 1 1 1 Ti j 1 _LiJ_ 1 1 mi 11 1 Jfc0 ° 1 2111 1 1 1 1 1 1 1 0 0 00000000000 1 0 1 1 1 1 1 1 1 Ti T 1 _bi_ 1 11 1 11 1 |2|00 12111 1 1 1 1 11 1 1 1 0 0 0 0000000000 1 0 sequence 1 ABCNJ RQCLCRPM A J C J N R C K C R B í" 1 1 1 1 1 1 1 1+5 3 2 2 0 0 3 3 3 4 3333433100 3 3 3 3 3 3 3 3 12 1 0 0 2 2 3 2 2 2 2 3 2 3 1 o|o 2 1 1 112 11 1 1 2 0 0 1 2 1 11111 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 OJ — a: A B C N J R Q c L C R P M A J C 8J7 66544332100 7^ 66644332 100 6 6* ^v6 5 4 4 4 3 3 1 0 0 J 6 6 6\5* ,44332 1 00 N S5S -^5 \4 4332100 R C 4 4 "3 3 4 4 4 3 4" 3 3 i 3 3 2 2 0 0 '4^,3 !3 1 Ó 0 K 3 3 3 3 3 3 3 3 "302 1 0 0 C 2 2 3 2 2 2 2 3 2f3^1 0 0 R 2 1 1 1 1 2 1 1 [1 1 " , 0 0 B 1 2 1 1 1 1 1111 l\0 0 P 0 0 0 0 0 0 0 0 0 0 0* 0 sequence 1 ABCNJ-RQCLCR-PM sequence 2 AJC-JNR-CKCRBP- sequence 1 ABC-NJRQCLCR-PM sequence 2 AJCJN-R-CKCRBP- Matice PAM 250 A 2 R -2 6 N 0 0 2 D Ü -1 2 4 (J -2 -4 -4 -5 12 Q Ü 1 1 2 -5 4 E 0 -1 1 3 -5 2 4 c; -3 Ü 1 -3 -1 Ü 5 H -1 2 2 1 -3 3 1 -2 6 i -1 -2 -2 -2 -2 -2 -2 -3 -2 5 L -2 -3 -3 -4 -6 -2 -3 -4 -2 -2 6 K -1 3 1 0 -5 1 0 -2 0 -2 -3 5 M -1 Ü -2 -3 -5 -1 -2 -3 -2 2 4 Ü 6 F -3 -4 -3 -6 -4 -5 -5 -5 -2 1 2 -5 Ü 9 P Ü Ü -1 -3 Ü -1 Ü Ü -2 -3 -1 -2 -5 6 S Ü 1 Ü Ü -1 Ü 1 -1 -1 -3 Ü -2 -3 1 2 T -1 Ü Ü -2 -1 Ü Ü -1 Ü -2 Ü -1 -3 Ü 1 3 W -6 2 -4 -7 -8 -5 -7 -7 -3 -5 -2 -3 -4 Ü -6 -2 -5 17 Y -3 -4 -2 -4 Ü -4 -4 -5 Ü -1 -1 -4 -2 7 -5 -3 -3 0 10 V Ü -2 -2 -2 -2 -2 -2 -1 -2 4 2 -2 2 -1 -1 -1 Ü -6 -2 4 A R N D C Q E G H 1 L K M F P S T W Y V Vícenásobné zarovnání prote n ů Snaha minimalizovat součet skóre všech dvojic S(v) = SUM(sm,sn), kde m=xQxKKLRT SEPDRKKF-fl TĽKERREYYS UTUQH|' J Xk/5 ■1,k )+S(xľ ,k-1)+S(xľ ,k )+S(xi; ■1,k-1)+S(-, xj5 xk), ■1,k )+S( -, Xj, xk), ,k-1)+S(-, -5xk)} Vícenásobné zarovnání protenů Metoda progresivní (funguje dobře pokud známe fylogenetické vztahy a začíname sekvencemi nqvíce příbuznými). Znánrý nástroj CLUSTALW ALGORITMUS: - přidq sekvenci - zarcvnq 2 sekvence - opakuj postup s dalšími sekvencemi Nástroje pro analýzu Predikce lokalizace Predikce sekundární struktury Predikce transmembráncvé topologie Predikce domén Predikce typu skládání Predikce struktury SIGNALP PSIPRED IVEMSAT2 CO/PRED GenTHREADER ROSETTA STRUKTURA -> FUNKCE ? It's a r est riet ion enzyme." Strukturní data PDB PDBsum i-,-..!- A Odvozená data SCOP (Class, Fold, Superfamily, Family) TI U barrel Sa-Kíwieh Roll (4lMl! | lir b A') CATH (Class, Architecture, lopology, Homologous superfamily) Strukturní data 1HEVV1AM7 LysQzym - enzym hydrolyzující (štěpící) vazbu mezi cukry pdysacharidů, které se nacházqí v buněčné stěně některých bakterií -zdroje: vqce, slzy, bakteriďág T4 Q/ü mm f- Jíw ě Wefy (~Jli« Wöifrl&fL AGERA LVSOÍTMAl-AntibaC For oily, acne-prone and problem skin conditions. • OIL-FREE • LYSOZYME / LIPOPEPTIDE ANTIMICROBIAL COMPLEX • MOISTURIZES BY SEALING IN MOISTURE • FRAGRANCE-FREE • CONTAINS ANTI-BACTERIAL ENZYME LYSOZYME PRICE: $20.00 http://www.rcsb.org/pdb/ PDB soubor - hlavička HEADER COMPND COMPND SOURCE AUTHOR REVDAT JRNL JRNL JRNL JRNL JRNL JRNL JRNL REMARK REMARK REMARK REMARK REMARK REMARK REMARK HYDROLASE(O-GLYCOSYL) 20-JAN-92 1HEW LYSOZYME (E.C.3.2.1.17) COMPLEXED WITH THE INHIBITOR 2 TRI-N-ACETYLCHITOTRIOSE HEN (GALLUS GALLUS) EGG WHITE J.C.CHEETHAM,P.J.ARTYMIUK,D.C.PHILLIPS 1 31-JAN-94 1HEW 0 AUTH J.C.CHEETHAM,P.J.ARTYMIUK,D.C.PHILLIPS TITL REFINEMENT OF AN ENZYME COMPLEX WITH INHIBITOR TITL 2 BOUND AT PARTIAL OCCUPANCY. HEN EGG-WHITE TITL 3 LYSOZYME AND TRI-N-ACETYLCHITOTRIOSE AT 1.75 TITL 4 ANGSTROMS RESOLUTION REF J.MOL.BIOL. V. 224 613 1992 REFN ASTM JMOBAK UK ISSN 0022-2836 070 1 1 1 1 1 1 1 REFERENCE 1 AUTH L.N.JOHNSON,J.C.CHEETHAM,P.J.MC*LAUGHLIN, AUTH 2 K.R.ACHARYA,D.BARFORD,D.C.PHILLIPS TITL PROTEIN-OLIGOSACCHARIDE INTERACTIONS: LYSOZYME, TITL 2 PHOSPHORYLASE, AMYLASES REF CURR.TOP.MICROBIOL.IMMUNOL. V. 139 81 1988 1HEW 2 1HEW 3 1HEW 4 1HEW 5 1HEW 6 1HEW 7 1HEW 8 1HEW 9 1HEW 10 1HEW 11 1HEW 12 1HEW 13 1HEW 14 1HEW 15 1HEW 16 1HEW 17 1HEW 18 1HEW 19 1HEW 20 1HEW 21 PDB soubor - primární struktura REMARK 5 THE TH REMARK 5 IN THE REMARK 5 SITE C REMARK 5 BOUND SEQRES 1 129 SEQRES 2 129 SEQRES 3 129 SEQRES 4 129 SEQRES 5 129 SEQRES 6 129 SEQRES 7 129 SEQRES 8 129 SEQRES 9 129 SEQRES 10 129 HET NAG 201 HET NAG 202 HET NAG 203 FORMÚL 2 NAG FORMÚL 3 HOH EE SUGAR UNITS OF THE INHIBITOR MOLECULE ARE BOUND UPPER THREE SITES (A TO C) OF THE LYSOZYME ACTIVE CLEFT. NAG MOLECULES, NUMBERED 203, 202, AND 201, ARE IN SITES A, B, AND C, RESPECTIVELY. LYS VAL PHE GLY ARG CYS GLU LEU ALA ALA ALA MET LYS ARG HIS GLY LEU ASP ASN TYR ARG GLY TYR SER LEU GLY ASN TRP VAL CYS ALA ALA LYS PHE GLU SER ASN PHE ASN THR GLN ALA THR ASN ARG ASN THR ASP GLY SER THR ASP TYR GLY ILE LEU GLN ILE ASN SER ARG TRP TRP CYS ASN ASP GLY ARG THR PRO GLY SER ARG ASN LEU CYS ASN ILE PRO CYS SER ALA LEU LEU SER SER ASP ILE THR ALA SER VAL ASN CYS ALA LYS LYS ILE VAL SER ASP GLY ASN GLY MET ASN ALA TRP VAL ALA TRP ARG ASN ARG CYS LYS GLY THR ASP VAL GLN ALA TRP ILE ARG GLY CYS ARG LEU 15 N-ACETYL-D-GLUCOSAMINE 14 N-ACETYL-D-GLUCOSAMINE 14 N-ACETYL-D-GLUCOSAMINE 3(C8 H15 Nl 06) *103(H2 01) 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 PDB soubor- HELIX 1 A ARG 5 HIS HELIX 2 B LEU 25 GLU HELIX 3 C CYS 80 LEU HELIX 4 D THR 89 ILE HELIX 5 E VAL 109 ASN SHEET 1 SI 2 LYS 1 PHE SHEET 2 SI 2 PHE 38 THR SHEET 1 S2 3 ALA 42 ASN SHEET 2 S2 3 SER 50 GL Y SHEET 3 S2 3 GLN 51 ' SER TURN 1 TI MET 12 HIS TURN 2 T2 LYS 13 GLY TURN 3 T3 LEU 17 TYR TURN 4 T4 ASN 19 GLY TURN 5 T5 TYR 20 TYR TURN 6 T6 SER 24 ASN TURN 7 T7 LEU 25 TRP TURN 8 T8 SER 36 ASN TURN 9 T9 ASN 46 GLY sekundární struktura 15 1 1HEW 75 35 1 1HEW 76 84 5 1HEW 77 98 1 1HEW 78 113 1 1HEW 79 3 0 1HEW 80 40 -1 N THR 40 0 LYS 1 1HEW 81 46 0 1HEW 82 54 -1 0 SER 50 N ASN 46 1HEW 83 60 -1 0 ILE 58 N TYR 53 1HEW 84 15 TYPE III 1HEW 85 16 TYPE I 1HEW 86 20 TYPE II 1HEW 87 22 DISTORTED TYPE II 1HEW 88 23 TYPE ľ 1HEW 89 27 TYPE III 1HEW 90 28 TYPE III 1HEW 91 39 TYPE Ilľ 1HEW 92 49 TYPE I 1HEW 93 PDB soubor - terciární struktura CRYSTl 78. ,860 78. 860 38.250 90.00 i 90.00 90.00 P 43 21 2 8 1HEW 113 ORIGX1 1.000000 0. ,000000 0.000000 i 0.00000 1HEW 114 ORIGX2 0.000000 1. ,000000 0.000000 i 0.00000 1HEW 115 ORIGX3 0.000000 0. ,000000 1.000000 i 0.00000 1HEW 116 SCALE1 0.012681 0. ,000000 0.000000 i 0.00000 1HEW 117 SCALE2 0.000000 0. ,012681 0.000000 i 0.00000 1HEW 118 SCALE3 0.000000 0. ,000000 0.026144 0.00000 1HEW 119 ATOM 1 N LYS 1 3.398 9.981 10.408 1.00 30.48 1HEW 120 ATOM 2 CA LYS 1 2.459 10.365 9.364 1.00 28.03 1HEW 121 ATOM 3 C LYS 1 2.458 11.880 9.149 1.00 21.93 1HEW 122 ATOM 4 0 LYS 1 2.481 12.672 10.100 1.00 14.10 1HEW 123 ATOM 5 CB LYS 1 1.026 9.935 9.695 1.00 30.54 1HEW 124 ATOM 6 CG LYS 1 0.028 10.169 8.558 1.00 37.93 1HEW 125 ATOM 7 CD LYS 1 -1.415 10.089 9.048 1.00 33.23 1HEW 126 ATOM 8 CE LYS 1 -2.357 10.822 8.082 1.00 32.17 1HEW 127 ATOM 9 NZ LYS 1 -3.661 10.090 8.025 1.00 31.92 1HEW 128 ATOM 10 N VAL 2 2.429 12.232 7.880 1.00 17.30 1HEW 129 ATOM 11 CA VAL 2 2.395 13.653 7.465 1.00 14.47 1HEW 130 ATOM 12 C VAL 2 0.977 13.868 6.903 1.00 17.58 1HEW 131 ATOM 13 0 VAL 2 0.642 13.368 5.826 1.00 32.65 1HEW 132 ATOM 14 CB VAL 2 3.533 14.012 6.536 1.00 22.88 1HEW 133 Cn3D http://www.ncbi.nlm.nih.gov/Structure/CN3D/cn3d.shtml RasMol http://www.umass.edu/microbio/rasmol Chime/Protein Explorer http://www.umass.edu/microbio/chime/explorer/preview.htm I ■B AA j> 1 Crystal h ft * Ä Phosphatidyl cholines taken from model lipid bilayers simulated by mk AĚ .£ T Ô Heller et aľ, J. m mw S ^^H ^H Phys. Chem. Jv 97:8343, 1993. A. ľ^ > 1 if j mäm Al rWf *> f Fluid Eric M art z with RasMol Swiss PDB Viewer http://www.expasy.org/spdbv VMD http ://www .ks .uiuc. edu/Research/vmd/ PyMol http://pymol.sourceforge.net/ Dílčí funkce proteinů Enzymy (katäyzátory, substrát se proměňuje v produkt, aktivní místo) # Interakce protán-protán Interakce protán-DNA Interakce protán-ligand # Transdukce signálu, regulace * Strukturní proteiny (vlákna, glykoproteiny) * Motory Gene Ontology Funkce genů/protánů jsou zjišťovány experimentálně a publikovány v časopisech. Terminologie není zdaleka jednoznačná: protán synthesis - translation - ribosomal complex - peptide chán elongation Ontológie jsou vytvářeny ve snaze zavést do popisu funkcí určitý systém Gene Ontology »definice ontológií -strukturovaných sad termínů (DAG) pro popis biologické funkce molekulární funkce - lokalizace j priraďovaní uzlů vontologiíchgenůnfVprotánům vytváření nástrojů pro využití dat jhttp://www.geneontology.org/ GO -ŕ" Structure of the GO is-a Dart-of ce nucleus membrane nuclear membrane molecular function nucleic acid binding I DNA binding I chromatin binding / MCM2 Mcml Mcmdl f MCM3 Mcm3 Mcmd \ CDC54/MCM4 Mcmd4 CDC46/MCM5 McmS MCM6 Mcw6 Mcmd6 CDC47/MCM7 Mcm7 Mcmd7 \ la m in/chroma tin binding SACCHAROMYCES DROSOPHILA MUS enzyme DNA helicase ATP-dependent helicase | hay mus309 I adenosine triphosphatase DNA-dependent adenosine triphosphatase Rad51 ATP-dependent DNA helicase MCM2 MCM3 CDC54/MCM4 Mcmd4 CDC46/MCM5 MCM6 Mcmd6 CDC47/MCM7 Mcmd7 Hierarchy Query for this ten Returns things annotated to descendents DAG molecular chapero chaperone activator Gene Ontology - ontológie id: GO:0006903 name: vesicle targeting namespace: biological_process def: "Targeting of a vesicle to a specific destination membrane." [GO:jic] relationship: part_of GO:0016192 ! vesicle-mediated transport [Term] id: GO:0006904 name: vesicle docking during exocytosis namespace: biological_process def: "The initial attachment of a vesicle membrane to a target membraneX, mediated by proteins protruding from the membrane of the vesicle and the target membraneX, during exocytosis." [GO:jic] subset: gosubset_prok is_a: GO:0048278 ! vesicle docking relationship: part_of GO:0006887 ! exocytosis [Term] id: GO:0006905 name: vesicle transport namespace: biological_process def: "OBSOLETE (was not defined before being made obsolete)." [GO:curators] comment: This term was made obsolete because the meaning of the term is ambiguous. To update annotationsX, consider the biological process term 'vesicle-mediated transport ; GO\:0016192'. is obsolete: true Gene Ontology - alternativní formát ontdogie %polyol catabolism ; GO:0046174 % polyol metabolism ; GO:0019751 %alditol catabolism ; GO:0019405 % alditol metabolism ; GO:0019400 %hexitol catabolism ; GO:0019407 % hexitol metabolism ; GO:0006059 %galactitol catabolism ; GO:0019404 %mannitol catabolism ; GO:0019592 %sorbitol catabolism ; GO:0006062 %pentitol catabolism ; GO:0019527 % pentitol metabolism ; GO:0019519 %arabitol catabolism ; GO:0051157 % arabitol metabolism ; GO:0051161 %arabitol utilization ; GO:0019591 %D-arabitol catabolism ; GO:0051159 % D-arabitol metabolism ; GO:0051163 %D-arabitol catabolism to xylulose 5-phosphate ; GO:0019528 Gene Ontology - anotace TAIR gene:1944535 ERS2 GO:0004673 ISS F ETHYLENE RESPONSE SENSOR 2 ETHYLENE RESPONSE SENSOR 2 gene taxon:3702 TAIR:Communication:1675000 ERS2 PROTEIN|AT1G04310| 20020827 TAIR TAIR gene:1944536 ETRl GO:0005783 TAIR:Publication:1547355| PMID:11916973 IDA C ETHYLENE RESPONSE 1 ETR|HISTIDINE KINASE ETRl|AT1G66340|EINl|ETHYLENE INSENSITIVE 1|ETHYLENE RESPONSE 1 gene taxon:3702 20020904 TAIR TAIR gene:1944536 ETRl GO:0009727 TAIR:Publication:1795| PMID:9974395 IMP P ETHYLENE RESPONSE 1 ETR|HISTIDINE KINASE ETRl|AT1G66340|EINl|ETHYLENE INSENSITIVE 1|ETHYLENE RESPONSE 1 gene taxon:3702 20020904 TAIR TAIR gene:1944536 ETRl GO:0004673 TAIR:Communication:1675000 ISS F ETHYLENE RESPONSE 1 ETR|HISTIDINE KINASE ETRl|AT1G66340| EINl|ETHYLENE INSENSITIVE 1|ETHYLENE RESPONSE 1 gene taxon:3702 20020827 TAIR TAIR gene:1944538 ETR2 GO:0004673 TAIR:Communication:1675000 ISS F ETHYLENE RESPONSE 2 ETHYLENE RESPONSE 2|AT3G23150|ETR2 gene taxon:3702 20020827 TAIR The Wellcome Trust Sanger lna bi tuto ,ie Scmzo$accnraromvces Domoe ienpme Seguenďma P/pjecí i lie Paťhoaen Group ■ bjtcí\Ba^e fe hé Mouse Gefiomv Informatics FlyBase GO ■^a GENE ONTOLOGY™ CONSORTIUM i: InterPro suiissprot Oepame í Knowfe<£eeBase s £S£STt\ IncyteGcfioiri CS Fffl-Liio. GRAMEN] rmgrtt ZFIN AstraZenecá WormBase J _| THE INSTITUTE FOR GENOMIC RESEARCH compugen RGD paired box gene 3 paired domain gene 3; PAX3/FKHR fusion gene; paired domain gene HuP2; paired box homeotic gene 3 Plays a critical role during fetal development. Mutations are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome and alveolar rhabdomyosarcoma. biologicaLprocess rnolecular.f unction cellular.cornponent physiological processes development binding transcription regulator activity nucleobase, nucleoside, nucleotide and nucleic acid morphogenesis metabolism nucleic acid binding intracellular DNA binding nucleus transcription transcription factor activity nucleoplasn inscription, DNA-dependent regulation of transcription organogenesis regulation of transcription DNA-dependent transcription factor complex 3 VLAD - VisuaL Annotation Display - Microsoft Internet Explorer JnJxJ File Edit VJew Favorites lools Help jBack ' =r "9 @ fl ŠftSearch 3J Favorites #Media 0 | %* S & T M I * ^ Address ^) http://proto,informaocs.jax.org/prototypes/vlad/ » í>Go Links J> íj * Trř^ Search Web - Eg'| B Mail - ® My Yahoo! Ig| Games - iff Yahoo! - ^Personals - t^ LAUNCH -[ Sign In~[* ! VLAD C 1.0 <■ ^ (T~"> f-i o «ä_^ <-> VLAD - VisuaL Annotation Display VLAD is a tool for visualizing GO annotations. The annotation data are a subset of those availab will produce a graphical summary of the annotations. Help Feedback (Some...) (problems? suggestions? requests?) Download (coming soc Vocabularies: Annotation Set: Annotations Filters: Query Set: (gene symbols and/or IDs, use textarea or upload file) I7 Biological Process. I? Molecular Function. I7 Cellular Component MGI Exclude IEA annotations. Using the GO for data analysis...is there a functional "theme" in your set of genes? d Upload file of symbols/IDs: Browse... Scoring: f Percentages C P-values Display Settings: http://proto.infornriatics.jax.org/prototypes/vlad/ |20 Collapsing threshold. Submit Query Reset gf]Done d Internet Funkce zastoupeny v naší sadě genů/proteinů častěji než by bylo možné očekávat na základě náhody jsou zvýrazněny zeleně. Biological Process 4 negative regulation í negative regulation of contraption______ í negative regulation of muscle contraction "implicit" terms actual GO terms ,i--.+i^^. regulation ▲ regulation of pontraption cell motility )art of contraction í \ regulation of muscle contraction ^^^ffi muscle contraction t regulation of smooth muscle pontraption__ í ^^^OT smooth muscle pontraption____ ^ffl ttim negative regulation of smooth musp.le pontraption AmiGO http://www.godatabase.org/ m AmiGO : G0:0000724 details - Netscape __ JSjxl ?rTi 7ž GOst Search Get this GO teirn as RDF XML Get this data as a GO flat file, double-strand break repair via homologous recombination Accession:GO:0000724 Synonyms: None, Definition: The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequenced The restoration of two intact DNA molecules results in the exchange, reciprocal or non-reciprocal, of genetic material between the intact Dl\~~ HTerm Lineage GO:0003673 : Gene Ontology (72737) ® GO:000S150 : biological process (49448) ®GO:0007582 : physiological processes (35542) ® GO:0008152 : metabolism f24141) (DGO:0006139 : nucleobase, nucleoside, nucleotide and nucleic acid metabolism (9104) ® GO:0006259 : DNA metabolism (2822) Cii GO:0006310 : DNA recombination (947) Cii GO:000Ď312 : mitotic recombination (52) GO:0000725 : recombinational repair (32) ® 00:0000724 : double-strand break repair via homologous recombination (32) ® GO:0006281 : DNA repair (733) ® GO:0006302 : double-strand break repair (77) 'X1 GQ:0Q00724 : double-strand break repair via homologous recombination (32) ® GO:0000725 : recombinational repair (32) 3) GO:0000724 : double-strand break repair via homologous recombination (32) Etxternal References None,__________________________________________ ElASSOCiated Genes Bene Filters: Filter by database: FlyBase SOD Filter by Evidence for Association: Page 1 LH íäl E3 cS ^Ťľ I Document! Done (5,208 sees) .=&=&