•Evolution and functions of SMC complexes: new SMC5/6 insights
•Fakulta informatiky, Brno, 18.10. 2016


Čím se u nás zabýváme
•Proteomika/biochemie
•Proteinové (bílkovinné) komplexy (složení, funkce, patogeneze, evoluce) •Zprostředkovány
protein-proteinovými interakcemi •Interakce SMC a NSE proteinů – utváří SMC5/6 komplex (a jiné SMC
komplexy)

SMC complexes
•SMC, kleisin and kleisin-associated proteiny (podjednotky)
•Konzervované od bakterií po člověka
•Smc/ScpAB or MukBEF (plus MksBEF)
•3 komplexy u člověka (cohesin, condensin, SMC5/6)
•Kleisin
•(long)
•Kleisin-interacting
•winged-helices
•Kleisin-interacting
•HEAT repeats
•Kleisin
•(short)

•ScpB (KITE = Kleisin Interacting Tandem-winged-helix Element) holds and shapes ScpA kleisin (PDB:
4I98; Gruber)
Bacterial KITE proteins shape kleisins
•ScpB (KITE) proteins interact through their WHA (first WHD)
•
•ScpB (KITE) proteins bind ScpA kleisin via second WH domain
•(WHB)
•
•KITEs shape kleisin’s middle part (regulate their binding to SMC ring?)
•Palecek and Gruber, Structure, 2015

SMC komplexy
•Structure Maintenance of Chromosome complexes form rings
•Rings can embrace DNA/chromatin fibers
•Form loops (higher order chromatin structure)
•Hold two sister chromatids (segregation, homologous recomb.)
•Assist in replication (fork progression and stability)
•Haering and Jessberger, Exp Cell Biol, 2012